Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing

Na Xu; Yucheng Yuan; Liangping Ding; Jiangfeng Li; Jiezhi Jia; Zheng Li; Dengfeng He; Yunlong Yu

doi:10.1093/burnst/tkac019

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Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing

更新时间：2023-09-05

- Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing
- Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing
- Burns & Trauma 2022年10卷第1期
- 作者机构：
  
  1. Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University)
  2. School of Materials science and Engineering, Xihua University, No.999, Jinzhou Road, Jinniu District, Chengdu City,Sichuan Province
- 作者简介：
- 基金信息：
  
  The study was supported by the National Natural Science Foundation of China (51703145), and Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0513).
- DOI：10.1093/burnst/tkac019
  中图分类号：
- 纸质出版日期：2022，
  
  网络出版日期：2022-7-27，
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Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing[J]. Burns & Trauma, 2022,10(1).

Na Xu, Yucheng Yuan, Liangping Ding, Jiangfeng Li, Jiezhi Jia, Zheng Li, Dengfeng He, Yunlong Yu, Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing, Burns & Trauma, Volume 10, 2022, tkac019, https://doi.org/10.1093/burnst/tkac019
Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing[J]. Burns & Trauma, 2022,10(1). DOI： 10.1093/burnst/tkac019.

Na Xu, Yucheng Yuan, Liangping Ding, Jiangfeng Li, Jiezhi Jia, Zheng Li, Dengfeng He, Yunlong Yu, Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing, Burns & Trauma, Volume 10, 2022, tkac019, https://doi.org/10.1093/burnst/tkac019 DOI：

摘要

Abstract

BackgroundMost traditional wound dressings only partially meet the needs of wound healing because of their single function. Patients usually suffer from the increasing cost of treatment and pain resulting from the frequent changing of wound dressings. Herein

we have developed a mutifunctional cryogel to promote bacterial infected wound healing based on a biocompatible polysaccharide.MethodsThe multifunctional cryogel is made up of a compositive scaffold of chitosan (CS)

gelatin (Gel) and tannic acid (TA) and in situ formed silver nanoparticles (Ag NPs). A liver bleeding rat model was used to evaluate the dynamic hemostasis performance of the various cryogels. In order to evaluate the antibacterial properties of the prepared cryogels

gram-positive bacterium Staphylococcus aureus (S. aureus) and gram-negative bacterium Escherichia coli (E. coli) were cultured with the cryogels for 12 h. Meanwhile

S. aureus was introduced to cause bacterial infection in vivo. After treatment for 2 days

the exudates from wound sites were dipped for bacterial colony culture. Subsequently

the anti-inflammatory effect of the various cryogels was evaluated by western blotting and enzyme-linked immunosorbent assay. Finally

full-thickness skin defect models on the back of SD rats were established to assess the wound healing performances of the cryogels.ResultsDue to its porous structure

the multifunctional cryogel showed fast liver hemostasis. The introduced Ag NPs endowed the cryogel with an antibacterial efficiency of >99.9% against both S. aureus and E. coli. Benefited from the polyphenol groups of TA

the cryogel could inhibit nuclear factor-κB nuclear translocation and down-regulate inflammatory cytokines for an anti-inflammatory effect. Meanwhile

excessive reactive oxygen species could also be scavenged effectively. Despite the presence of Ag NPs

the cryogel did not show cytotoxicity and hemolysis. Moreover

in vivo experiments demonstrated that the biocompatible cryogel displayed effective bacterial disinfection and accelerated wound healing.ConclusionsThe multifunctional cryogel

with fast hemostasis

antibacterial and anti-inflammation properties and the ability to promote cell proliferation could be widely applied as a wound dressing for bacterial infected wound healing.

关键词

Keywords

Wound dressingCryogelBacterial infectionAnti-inflammationHemostasisAntibacterialWound healing

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