Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance

Weiguo Xie; Weigang Hu; Zhuo Huang; Min Li; Hongyu Zhang; Xiaodong Huang; Paul Yao

doi:10.1093/burnst/tkac007

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Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance

更新时间：2022-06-09

- Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance
- Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance
- Burns & Trauma 2022年10卷第1期
- 作者机构：
  
  1. Institute of Burns, Tongren Hospital of Wuhan University (Wuhan Third Hospital)
  2. Department of Hematology
  3. Peking University Shenzhen Hospital
- 作者简介：
- 基金信息：
  
  This study was financially supported by The National Natural Science Foundation of China, Project #: 81772097 and Revival Program of Growth Factors, Project #: SZYZ-TR-10.
- DOI：10.1093/burnst/tkac007
  中图分类号：
- 纸质出版日期：2022，
  
  网络出版日期：2022-4-9，
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Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance[J]. Burns & Trauma, 2022,10(1).

Weiguo Xie, Weigang Hu, Zhuo Huang, Min Li, Hongyu Zhang, Xiaodong Huang, Paul Yao, Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance, Burns & Trauma, Volume 10, 2022, tkac007, https://doi.org/10.1093/burnst/tkac007
Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance[J]. Burns & Trauma, 2022,10(1). DOI： 10.1093/burnst/tkac007.

Weiguo Xie, Weigang Hu, Zhuo Huang, Min Li, Hongyu Zhang, Xiaodong Huang, Paul Yao, Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance, Burns & Trauma, Volume 10, 2022, tkac007, https://doi.org/10.1093/burnst/tkac007 DOI：

摘要

Abstract

BackgroundDiabetes significantly delays wound healing through oxidative stress

inflammation and impaired re-epithelialization that lead to defective regulation of the healing process

although the related mechanism remains unclear. Here

we aim to investigate the potential role and mechanism for the beneficial effect of betulinic acid (BA) on diabetic wound healing.MethodsThe molecular effect of BA on hyperglycemia-mediated gene expression

oxidative stress

inflammation and glucose uptake was evaluated in endothelial

fibroblast and muscle cells. Burn injury was introduced to streptozotocin-induced diabetic rats and BA administration through either an intraperitoneal (IP) or topical (TOP) technique was used for wound treatment. Glucose tolerance was evaluated in both muscle tissue and fibroblasts

while oxidative stress and inflammation were determined in both the circulatory system and in wound tissues. The effect of BA on the wound healing process was also evaluated.ResultsBA treatment reversed hyperglycemia-induced glucose transporter type 4 (GLUT4) suppression in both muscle and fibroblast cells. This treatment also partly reversed hyperglycemia-mediated suppression of endothelial nitric oxide synthase (eNOS)

nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and nuclear factor NFκB p65 subunit (NFκB p65) activation in endothelial cells. An in vivo rat study showed that BA administration ameliorated diabetes-mediated glucose intolerance and partly attenuated diabetes-mediated oxidative stress and inflammation in both the circulatory system and wound tissues. BA administration by both IP and TOP techniques significantly accelerated diabetic wound healing

while BA administration by either IP or TOP methods alone had a significantly lower effect.ConclusionsBA treatment ameliorates hyperglycemia-mediated glucose intolerance

endothelial dysfunction

oxidative stress and inflammation. Administration of BA by both IP and TOP techniques was found to significantly accelerate diabetic wound healing

indicating that BA could be a potential therapeutic candidate for diabetic wound healing.

关键词

Keywords

Betulinic aciddiabetesInflammationOxidative stressWound healingNuclear factor κBglucoseHyperglycemia

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