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Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds
Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds
- Burns & Trauma 2022年10卷第1期
- 作者机构：
1. Second Affiliated Hospital of Zhejiang University College of Medicine
2. The Second Affiliated Hospital of Zhejiang University College of Medicine
- 作者简介：
- 基金信息：This work was supported by Grants from Zhejiang Provincial Basic Public Welfare Research Program (LGF19H150008) and the Natural Science Foundation of Zhejiang province (LGF20H150004).
DOI：10.1093/burnst/tkac001
中图分类号：纸质出版日期：2022，
网络出版日期：2022-3-14，
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Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds[J]. Burns & Trauma, 2022,10(1).
Sizhan Xia, Tingting Weng, Ronghua Jin, Min Yang, Meirong Yu, Wei Zhang, Xingang Wang, Chunmao Han, Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds, Burns & Trauma, Volume 10, 2022, tkac001, https://doi.org/10.1093/burnst/tkac001
Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds[J]. Burns & Trauma, 2022,10(1). DOI： 10.1093/burnst/tkac001.
Sizhan Xia, Tingting Weng, Ronghua Jin, Min Yang, Meirong Yu, Wei Zhang, Xingang Wang, Chunmao Han, Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds, Burns & Trauma, Volume 10, 2022, tkac001, https://doi.org/10.1093/burnst/tkac001 DOI：
摘要
Abstract
BackgroundGelatin methacryloyl (GelMA) hydrogels loaded with stem cells have proved to be an effective clinical treatment for wound healing. Advanced glycation end product (AGE)
interacting with its particular receptor (AGER)
gives rise to reactive oxygen species (ROS) and apoptosis. Curcumin (Cur) has excellent antioxidant activity and regulates intracellular ROS production and apoptosis. In this study
we developed a Cur-incorporated 3D-printed GelMA to insert into adipose-derived stem cells (ADSCs) and applied it to diabetic wounds.MethodsGelMA hydrogels with Cur were fabricated and their in vitro effects on ADSCs were investigated. We used structural characterization
western blot
ROS and apoptosis assay to evaluate the antioxidant and anti-apoptotic activity
and assessed the wound healing effects to investigate the mechanism underlying regulation of apoptosis by Cur via the AGE/AGER/nuclear factor-κB (NF-κB) p65 pathway.ResultsA 10% GelMA scaffold exhibited appropriate mechanical properties and biocompatibility for ADSCs. The circular mesh structure demonstrated printability of 10% GelMA and Cur-GelMA bioinks. The incorporation of Cur into the 10% GelMA hydrogel showed an inhibitory effect on AGEs/AGER/NF-κB p65-induced ROS generation and ADSC apoptosis. Furthermore
Cur-GelMA scaffold promoted cell survival and expedited in vivo diabetic wound healing.ConclusionsThe incorporation of Cur improved the antioxidant activity of 3D-printed GelMA hydrogel and mitigated AGE/AGER/p65 axis-induced ROS and apoptosis in ADSCs. The effects of scaffolds on wound healing suggested that Cur/GelMA-ADSC hydrogel could be an effective biological material for accelerating wound healing.
关键词
Keywords
Curcumin3D printingGelMAAdvanced glycation end productsAdipose-derived stem cellsWound healing
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