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Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing
Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing
- Burns & Trauma 2021年9卷第1期
- 作者机构：
1. Department of Biology & Guangdong Provincial Key Laboratory of Marine Biotechnology
2. Shantou University
3. Department of Plastic Surgery and Burn Center
4. Plastic Surgery Institute of Shantou University Medical College
- 作者简介：
- 基金信息：This work is supported by the National Natural Science Foundation of China (No. 2147613), the Educational Commission of Guangdong Province, China (2021ZDZX2051), Li Ka Shing Foundation Cross-Disciplinary Research Grant (Project Number: 2020LKSFG02E and 2020LKSFG18B).
DOI：10.1093/burnst/tkab041
中图分类号：纸质出版日期：2021，
网络出版日期：2021-12-22，
扫 描 看 全 文
Huanhuan Wang, Yang Liu, Kun Cai, 等. Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing[J]. Burns & Trauma, 2021,9(1).
Huanhuan Wang, Yang Liu, Kun Cai, Bin Zhang, Shijie Tang, Wancong Zhang, Wenhua Liu, Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing, Burns & Trauma, Volume 9, 2021, tkab041, https://doi.org/10.1093/burnst/tkab041
Huanhuan Wang, Yang Liu, Kun Cai, 等. Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing[J]. Burns & Trauma, 2021,9(1). DOI： 10.1093/burnst/tkab041.
Huanhuan Wang, Yang Liu, Kun Cai, Bin Zhang, Shijie Tang, Wancong Zhang, Wenhua Liu, Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing, Burns & Trauma, Volume 9, 2021, tkab041, https://doi.org/10.1093/burnst/tkab041 DOI：
摘要
Abstract
BackgroundPolysaccharide-based hydrogels have been developed for many years to treat burn wounds. Essential oils extracted from aromatic plants generally exhibit superior biological activity
especially antibacterial properties. Studies have shown that antibacterial hydrogels mixed with essential oils have great potential for burn wound healing. This study aimed to develop an antibacterial polysaccharide-based hydrogel with essential oil for burn skin repair.MethodsEucalyptus essential oil (EEO)
ginger essential oil (GEO) and cumin essential oil (CEO) were employed for the preparation of effective antibacterial hydrogels physically crosslinked by carboxymethyl chitosan (CMC) and carbomer 940 (CBM). Composite hydrogels were prepared and characterized using antimicrobial activity studies
Fourier-transform infrared spectroscopy
X-ray diffraction
scanning electron microscopy
gas chromatography-mass spectrometery
rheological analysis
viscosity
swelling
water loss rate and water vapor transmission rate studies. In addition
the biocompatibility of hydrogels was evaluated in vivo by cytotoxicity and cell migration assays and the burn healing ability of hydrogels was tested in vivo using burn-induced wounds in mice.ResultsThe different essential oils exhibited different mixing abilities with the hydrogel matrix (CMC and CBM)
which caused varying levels of reduction in essential oil hydrogel viscosity
swelling and water vapor transmission. Among the developed hydrogels
the CBM/CMC/EEO hydrogel exhibited optimal antibacterial activities of 46.26 ± 2.22% and 63.05 ± 0.99% against Staphylococcus aureus and Escherichia coli
respectively
along with cell viability (>92.37%) and migration activity. Furthermore
the CBM/CMC/EEO hydrogel accelerated wound healing in mouse burn models by promoting the recovery of dermis and epidermis as observed using a hematoxylin–eosin and Masson’s trichrome staining assay. The findings from an enzyme-linked immunosorbent assay demonstrated that the CBM/CMC/EEO hydrogel could repair wounds through interleukin-6 and tumor necrosis factor-α downregulation and transforming growth factor-β
vascular endothelial growth factor (VEGF) and epidermal growth factor upregulation.ConclusionsThis study successfully prepared a porous CBM/CMC/EEO hydrogel with high antibacterial activity
favorable swelling
optimal rheological properties
superior water retention and water vapor transmission performance and a significant effect on skin repair in vitro and in vivo. The results indicate that the CBM/CMC/EEO hydrogel has the potential for use as a promising burn dressing material for skin burn repair.
关键词
Keywords
Carboxymethyl chitosanEucalyptus essential oilHydrogelAntibacterial activityBurnWound healing
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