Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing

Xiaoxuan Lei; Liuhanghang Cheng; Yu Yang; Mengru Pang; Yunqing Dong; Xuanru Zhu; Caihong Chen; Zexin Yao; Gang Wu; Biao Cheng; Tymour Forouzanfar

doi:10.1093/burnst/tkab033

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Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing

更新时间：2022-06-14

- Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing
- Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing
- Burns & Trauma 2021年9卷第1期
- 作者机构：
  
  1. Department of Oral and Maxillofacial Surgery/Pathology
  2. Vrije Universiteit Amsterdam, Amsterdam Movement Science
  3. Department of Burn and Plastic Surgery
  4. General Hospital of Southern Theater Command
  5. Department of Oral Implantology and Prosthetic Dentistry
  6. University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU)
- 作者简介：
- 基金信息：
  
  This research was funded by National Natural Science Foundation of China, grant number 81671924 and 81272105;National Key Research and Development Plan of China, grant number 2017YFC1103301;Health and Medical Treatment Collaborative Innovation Major Special Projects of Guangzhou, grant number 201508020253;Science and Technology Key Project of Guangdong Province, grant number 2014B020212010;Science and Technology Planning Project of Guangdong Province of China, grant number 2015B020233012;Military Medical Innovation Special Projects, grant number 18CXZ029;Zhejiang Provincial Basic Public Welfare Research Project, grant number GJ19H140001;and China’s National Key R&D Programs (NKPs), grant number 2018YFB0407204.
- DOI：10.1093/burnst/tkab033
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-11-30，
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Xiaoxuan Lei, Liuhanghang Cheng, Yu Yang, 等. Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing[J]. Burns & Trauma, 2021,9(1).

Xiaoxuan Lei, Liuhanghang Cheng, Yu Yang, Mengru Pang, Yunqing Dong, Xuanru Zhu, Caihong Chen, Zexin Yao, Gang Wu, Biao Cheng, Tymour Forouzanfar, Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing, Burns & Trauma, Volume 9, 2021, tkab033, https://doi.org/10.1093/burnst/tkab033
Xiaoxuan Lei, Liuhanghang Cheng, Yu Yang, 等. Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing[J]. Burns & Trauma, 2021,9(1). DOI： 10.1093/burnst/tkab033.

Xiaoxuan Lei, Liuhanghang Cheng, Yu Yang, Mengru Pang, Yunqing Dong, Xuanru Zhu, Caihong Chen, Zexin Yao, Gang Wu, Biao Cheng, Tymour Forouzanfar, Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing, Burns & Trauma, Volume 9, 2021, tkab033, https://doi.org/10.1093/burnst/tkab033 DOI：

摘要

Abstract

BackgroundAcute skin wounds may compromise the skin barrier

posing a risk of infection. Small intestinal submucosa (SIS) is widely used to treat acute and chronic wounds. However

the efficacy of SIS to accelerate wound healing still needs to be improved to meet clinical demands. To tackle this problem

platelet-rich plasma (PRP) is used due to its potency to promote proliferation

migration and adhesion of target cells. In this study

we applied PRP and SIS to skin wounds to explore their effects on wound healing by evaluating re-epithelialization

collagen production

angiogenesis and the inflammatory response.MethodsA 1 × 1-cm full-thickness skin defect was established in mice. Sixty mice were divided into four treatment groups: PRP + SIS

PRP

SIS and control. On days 3

10 and 14 post-surgery

tissue specimens were harvested. Haematoxylin and eosin

Masson’s trichrome

immunohistochemical and immunofluorescence double staining were used to visualize epidermal thickness

collagen and vascular regeneration and inflammation.ResultsWound contraction in the PRP and PRP + SIS groups was significantly greater

compared with the other groups

on days 3 and 5 post-surgery. A histological analysis showed higher collagen expression in the PRP and PRP + SIS groups on day 7

which was associated with a thicker epidermal layer on day 14. In addition

immunohistochemical staining showed that CD31-positive blood vessels and vascular endothelial growth factor expression in the PRP + SIS and PRP groups were significantly higher

compared with the control group. Furthermore

immunofluorescence double staining showed that the number of M1 and M2 macrophages in the PRP + SIS and PRP groups was higher

compared with the control and SIS groups alone

on day 3. However

on day 7

the number of M1 macrophages dramatically decreased in the PRP + SIS and PRP groups. The ratio of M2 to M1 macrophages in the PRP + SIS and PRP groups was 3.97 and 2.93 times that of the control group and 4.56 and 3.37 times that of the SIS group

respectively.ConclusionCo-administration of SIS and PRP has a better effect on promoting angiogenesis

re-epithelialization and collagen regeneration in managing acute wound healing than either agent alone.

关键词

Keywords

Small intestinal submucosaPlatelet-rich plasmaFull-thickness skin defectM1 macrophagesM2 macrophagesAnimal modelWound healingskin

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