Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds

Maryam Azam; Hafiz Ghufran; Hira Butt; Azra Mehmood; Ramla Ashfaq; Asad M Ilyas; Muhammad R Ahmad; Sheikh Riazuddin

doi:10.1093/burnst/tkab021

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Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds

更新时间：2022-06-14

- Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds
- Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds
- Burns & Trauma 2021年9卷第1期
- 作者机构：
  
  1. National Centre of Excellence in Molecular Biology
  2. Jinnah Burn and Reconstructive Surgery Centre
- 作者简介：
- 基金信息：
  
  This work was financially supported by research grants from the Higher Education Commission of Pakistan.
- DOI：10.1093/burnst/tkab021
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-9-11，
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Maryam Azam, Hafiz Ghufran, Hira Butt, 等. Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds[J]. Burns & Trauma, 2021,9(1).

Maryam Azam, Hafiz Ghufran, Hira Butt, Azra Mehmood, Ramla Ashfaq, Asad M Ilyas, Muhammad R Ahmad, Sheikh Riazuddin, Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds, Burns & Trauma, Volume 9, 2021, tkab021, https://doi.org/10.1093/burnst/tkab021
Maryam Azam, Hafiz Ghufran, Hira Butt, 等. Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds[J]. Burns & Trauma, 2021,9(1). DOI： 10.1093/burnst/tkab021.

Maryam Azam, Hafiz Ghufran, Hira Butt, Azra Mehmood, Ramla Ashfaq, Asad M Ilyas, Muhammad R Ahmad, Sheikh Riazuddin, Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds, Burns & Trauma, Volume 9, 2021, tkab021, https://doi.org/10.1093/burnst/tkab021 DOI：

摘要

Abstract

BackgroundFollowing recent findings from our group that curcumin preconditioning augments the therapeutic efficacy of adipose-derived stem cells in the healing of diabetic wounds in rats

we aimed to investigate the regenerative effects of curcumin preconditioned adipose-derived mesenchymal stem cells (ASCs) for better recovery of acid inflicted burns in this study.MethodsASCs were preconditioned with 5 μM curcumin for 24 hours and assessed for proliferation

migration

paracrine release potential and gene expression comparative to naïve ASCs. Subsequently

the healing capacity of curcumin preconditioned ASCs (Cur-ASCs) versus naïve ASCs was examined using acidic wounds in rats. For this

acid inflicted burns of 20 mm in diameter were made on the back of male Wistar rats. Then

2 × 106 cells of Cur-ASCs and naïve ASCs were intradermally injected in the wound periphery (n = 6) for comparison with an untreated saline control. Post-transplantation

wounds were macroscopically analysed and photographed to evaluate the percentage of wound closure and period of re-epithelization. Healed wound biopsies were excised and used for histological evaluation and expression analysis of wound healing markers at molecular level by quantitative PCR and western blotting.ResultsWe found that Cur-ASCs exhibited greater proliferation

migration and paracrine potential in vitro. Further

Cur-ASCs showed more effective recovery than naïve ASCs as exhibited by gross morphology

faster wound closure and earlier re-epithelialization. Masson’s trichrome and hematoxylin and eosin staining demonstrated the improved architecture of the healing burns

as evidenced by reduced infiltration of inflammatory cells

compact collagen and marked granulation in Cur-ASC treated rats. Corroborating these findings

molecular assessment showed significantly reduced expressions of pro-inflammatory factors (interleukin-1 beta

interleukin-6

tumor necrosis factor alpha) a with striking upsurge of an oxidative marker (superoxide dismutase 1)

pro-angiogenic factors (vascular endothelial growth factor

hepatocyte growth factor

hypoxia-inducible factor-1 alpha) and collagen markers (transforming growth factor beta 1

fibroblast growth factor-2

collagen type 1 alpha 1)

verifying that Cur-ASCs modulate the regulation of pro-inflammatory and healing markers at burn sites.ConclusionsTreatment with Cur-ASCs resulted in faster re-epithelization of acid inflicted burns compared to the treatment with naïve ASCs. Based on observed findings

we suggest the transplantation of Cur-ASCs is a valuable therapy for the potent clinical management of acidic burns.

关键词

Keywords

Adipose-derived stem cellsCurcumin preconditioningAcidic burnWound healing

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