Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles

Yijie Zhang; Enhejirigala; Bin Yao; Zhao Li; Wei Song; Jianjun Li; Dongzhen Zhu; Yuzhen Wang; Xianlan Duan; Xingyu Yuan; Sha Huang; Xiaobing Fu

doi:10.1093/burnst/tkab013

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Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles

更新时间：2022-06-14

- Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles
- Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles
- Burns & Trauma 2021年9卷第1期
- 作者机构：
  
  1. Research Center for Tissue Repair and Regeneration
  2. Chinese PLA General Hospital and PLA Medical College
  3. PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury
  4. College of Graduate, Tianjin Medical University
  5. Institute of Basic Medical Research, Inner Mongolia Medical University
  6. The Shenzhen Key Laboratory of Health Sciences and Technology
  7. Tsinghua University
  8. Department of General Surgery
  9. Chinese PLA General Hospital
  10. Department of Burn and Plastic Surgery
  11. School of Medicine, Nankai University
  12. Research Unit of Trauma Care
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkab013
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-5-4，
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Yijie Zhang, Enhejirigala, Bin Yao, 等. Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles[J]. Burns & Trauma, 2021,9(1).

Yijie Zhang, Enhejirigala, Bin Yao, Zhao Li, Wei Song, Jianjun Li, Dongzhen Zhu, Yuzhen Wang, Xianlan Duan, Xingyu Yuan, Sha Huang, Xiaobing Fu, Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles, Burns & Trauma, Volume 9, 2021, tkab013, https://doi.org/10.1093/burnst/tkab013
Yijie Zhang, Enhejirigala, Bin Yao, 等. Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles[J]. Burns & Trauma, 2021,9(1). DOI： 10.1093/burnst/tkab013.

Yijie Zhang, Enhejirigala, Bin Yao, Zhao Li, Wei Song, Jianjun Li, Dongzhen Zhu, Yuzhen Wang, Xianlan Duan, Xingyu Yuan, Sha Huang, Xiaobing Fu, Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles, Burns & Trauma, Volume 9, 2021, tkab013, https://doi.org/10.1093/burnst/tkab013 DOI：

摘要

Abstract

BackgroundSweat glands (SGs) and hair follicles (HFs) are two important cutaneous appendages that play crucial roles in homeostatic maintenance and thermoregulation

and their interaction is involved in wound healing. SGs can be regenerated from mesenchymal stem cell-laden 3D bioprinted scaffolds

based on our previous studies

whereas regeneration of HFs could not be achieved in the same model. Due to the lack of an in vitro model

the underlying molecular mechanism of the interaction between SGs and HFs in regeneration could not be fully understood. The purpose of the present study was to establish an in vitro model of skin constructs with SGs and HFs and explore the interaction between these two appendages in regeneration.MethodsTo investigate the interaction effects between SGs and HFs during their regeneration processes

a combined model was created by seeding HF spheroids on 3D printed SG scaffolds. The interaction between SG scaffolds and HF spheroids was detected using RNA expression and immunofluorescence staining. The effects of microenvironmental cues on SG and HF regeneration were analysed by altering seed cell types and plantar dermis homogenate in the scaffold.ResultsAccording to this model

we overcame the difficulties in simultaneously inducing SG and HF regeneration and explored the interaction effects between SG scaffolds and HF spheroids. Surprisingly

HF spheroids promoted both SG and HF differentiation in SG scaffolds

while SG scaffolds promoted SG differentiation but had little effect on HF potency in HF spheroids. Specifically

microenvironmental factors (plantar dermis homogenate) in SG scaffolds effectively promoted SG and HF genesis in HF spheroids

no matter what the seed cell type in SG scaffolds was

and the promotion effects were persistent.ConclusionsOur approach elucidated a new model for SG and HF formation in vitro and provided an applicable platform to investigate the interaction between SGs and HFs in vitro. This platform might facilitate 3D skin constructs with multiple appendages and unveil the spatiotemporal molecular program of multiple appendage regeneration.

关键词

Keywords

Skin regenerationSweat glandsHair follicle3D bioprintingSpheroid cultureSkin constructs

references

1.Quinby WC Jr, Burke JF, Bondoc CC. Primary excision and immediate wound closure. Intensive Care Med. 1981;7:71–6.

2.Wang Y, Beekman J, Hew J, Jackson S, Issler-Fisher AC, Parungao R, et al. Burn injury: challenges and advances in burn wound healing, infection, pain and scarring. Adv Drug Deliv Rev. 2018;123:3–17.

3.Kim HS, Sun X, Lee JH, Kim HW, Fu X, Leong KW. Advanced drug delivery systems and artificial skin grafts for skin wound healing. Adv Drug Deliv Rev. 2019;146:209–39.

4.Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nat Biotechnol. 2014;32:773–85.

5.Zhou F, Hong Y, Liang R, Zhang X, Liao Y, Jiang D, et al. Rapid printing of bio-inspired 3D tissue constructs for skin regeneration. Biomaterials. 2020;258:120287. doi: 10.1016/j.biomaterials.2020.120287.

6.Noor N, Shapira A, Edri R, Gal I, Wertheim L, Dvir T. 3D printing of personalized thick and perfusable cardiac patches and hearts. Adv Sci (Weinh). 2019;6:1900344. doi: 10.1002/advs.201900344.

7.Varkey M, Visscher DO, van Zuijlen PPM, Atala A, Yoo JJ. Skin bioprinting: the future of burn wound reconstruction? Burns Trauma. 2019;7:4. doi: 10.1186/s41038-019-0142-7.

8.Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics. 2020;12. doi: 10.3390/pharmaceutics12080735.

9.Diao J, Liu J, Wang S, Chang M, Wang X, Guo B, et al. Sweat gland organoids contribute to cutaneous wound healing and sweat gland regeneration. Cell Death Dis. 2019;10:238. doi: 10.1038/s41419-019-1485-5.

10.Lee LF, Jiang TX, Garner W, Chuong CM. A simplified procedure to reconstitute hair-producing skin. Tissue Eng Part C Methods. 2011;17:391–400.

11.Lu C, Fuchs E. Sweat gland progenitors in development, homeostasis, and wound repair. Cold Spring Harb Perspect Med. 2014;4. doi: 10.1101/cshperspect.a015222.

12.Wang X, Tredget EE, Wu Y. dynamic signals for hair follicle development and regeneration. Stem Cells Dev. 2012;21:7–18.

13.Yao B, Wang R, Wang Y, Zhang Y, Hu T, Song W, et al. Biochemical and structural cues of 3D-printed matrix synergistically direct MSC differentiation for functional sweat gland regeneration. Sci Adv. 2020;6, 10:eaaz1094. doi: 10.1126/sciadv.aaz1094.

14.Lei M, Schumacher LJ, Lai YC, Juan WT, Yeh CY, Wu P, et al. Self-organization process in newborn skin organoid formation inspires strategy to restore hair regeneration of adult cells. Proc Natl Acad Sci U S A. 2017;114:E7101–10.

15.Lu CP, Polak L, Keyes BE, Fuchs E. Spatiotemporal antagonism in mesenchymal-epithelial signaling in sweat versus hair fate decision. Science. 2016;354:aah6102. doi: 10.1126/science.aah6102.

16.Pati F, Jang J, Ha DH, Won Kim S, Rhie JW, Shim JH, et al. Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun. 2014;5:3935. doi: 10.1038/ncomms4935.

17.Lee CH, Rodeo SA, Fortier LA, Lu C, Erisken C, Mao JJ. Protein-releasing polymeric scaffolds induce fibrochondrocytic differentiation of endogenous cells for knee meniscus regeneration in sheep. Sci Transl Med. 2014;6:266ra171. doi: 10.1126/scitranslmed.3009696.

18.Ma Y, Lin M, Huang G, Li Y, Wang S, Bai G, et al. 3D spatiotemporal mechanical microenvironment: a hydrogel-based platform for guiding stem cell fate. Adv Mater. 2018;30:e1705911. doi: 10.1002/adma.201705911.

19.West-Livingston LN, Park J, Lee SJ, Atala A, Yoo JJ. The role of the microenvironment in controlling the fate of bioprinted stem cells. Chem Rev. 2020;120:11056–92.

20.Wang R, Wang Y, Yao B, Hu T, Li Z, Liu Y, et al. Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment. Burns Trauma. 2019;7:29. doi: 10.1186/s41038-019-0167-y.

21.Abaci HE, Coffman A, Doucet Y, Chen J, Jackow J, Wang E, et al. Tissue engineering of human hair follicles using a biomimetic developmental approach. Nat Commun. 2018;9:5301. doi: 10.1038/s41467-018-07579-y.

22.Chen R, Zhu Z, Ji S, Geng Z, Hou Q, Sun X, et al. Sweat gland regeneration: current strategies and future opportunities. Biomaterials. 2020;255:120201. doi: 10.1016/j.biomaterials.2020.120201.

23.Ito M, Yang Z, Andl T, Cui C, Kim N, Millar SE, et al. Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature. 2007;447:316–20.

24.Lu CP, Polak L, Rocha AS, Pasolli HA, Chen SC, Sharma N, et al. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell. 2012;150:136–50.

25.Cui C-Y, Ishii R, Campbell DP, Michel M, Piao Y, Kume T, et al. Foxc1 ablated mice are anhidrotic and recapitulate features of human miliaria sweat retention disorder. J Invest Dermatol. 2017;137:38–45.

26.Tong X, Coulombe PA. Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion. Genes Dev. 2006;20:1353–64.

27.Shimomura Y, Wajid M, Shapiro L, Christiano AM. P-cadherin is a p63 target gene with a crucial role in the developing human limb bud and hair follicle. Development. 2008;135:743–53.

28.Fuchs E. Scratching the surface of skin development. Nature. 2007;445:834–42.

29.Meng F, Meyer CM, Joung D, Vallera DA, McAlpine MC, Panoskaltsis-Mortari A. 3D bioprinted in vitro metastatic models via reconstruction of tumor microenvironments. Adv Mat. 2019;31:e1806899. doi: 10.1002/adma.201806899.

30.Yan WC, Davoodi P, Vijayavenkataraman S, Tian Y, Ng WC, Fuh JYH, et al. 3D bioprinting of skin tissue: from pre-processing to final product evaluation. Adv Drug Deliv Rev. 2018;132:270–95.

31.Wang X, Chen H, Tian R, Zhang Y, Drutskaya MS, Wang C, et al. Macrophages induce AKT/beta-catenin-dependent Lgr5(+) stem cell activation and hair follicle regeneration through TNF. Nat Commun. 2017;8:14091. doi: 10.1038/ncomms14091.

32.Huang S, Yao B, Xie J, Fu X. 3D bioprinted extracellular matrix mimics facilitate directed differentiation of epithelial progenitors for sweat gland regeneration. Acta Biomater. 2016;32:170–7.

33.Huang S, Xu Y, Wu C, Sha D, Fu X. In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands. Biomaterials. 2010;31:5520–5.

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