Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization

Zengjun Yang; Xiaohong Hu; Lina Zhou; Yaxiong He; Xiaorong Zhang; Jiacai Yang; Zhenyu Ju; Yih-Cherng Liou; Han-Ming Shen; Gaoxing Luo; Michael R Hamblin; Weifeng He; Rui Yin

doi:10.1093/burnst/tkab008

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Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization

更新时间：2022-06-14

- Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization
- Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization
- Burns & Trauma 2021年9卷第1期
- 作者机构：
  
  1. Department of Dermatology
  2. Southwest Hospital, Third Military Medical University (Army Medical University)
  3. State Key Laboratory of Trauma
  4. Chongqing Key Laboratory for Disease Proteomics
  5. Department of Endocrinology
  6. Key Laboratory of Regenerative Medicine of Ministry of Education
  7. Jinan University
  8. Department of Biological Sciences
  9. National University of Singapore
  10. Department of Physiology
  11. Yong Loo Lin School of Medicine, National University of Singapore
  12. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School
  13. Laser Research Centre
  14. University of Johannesburg
- 作者简介：
- 基金信息：
  
  This work was supported by National Natural Science Foundation of China (grant No. 81571902 and 31872742).
- DOI：10.1093/burnst/tkab008
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-9-6，
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Zengjun Yang, Xiaohong Hu, Lina Zhou, 等. Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization[J]. Burns & Trauma, 2021,9(1).

Zengjun Yang, Xiaohong Hu, Lina Zhou, Yaxiong He, Xiaorong Zhang, Jiacai Yang, Zhenyu Ju, Yih-Cherng Liou, Han-Ming Shen, Gaoxing Luo, Michael R Hamblin, Weifeng He, Rui Yin, Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization, Burns & Trauma, Volume 9, 2021, tkab008, https://doi.org/10.1093/burnst/tkab008
Zengjun Yang, Xiaohong Hu, Lina Zhou, 等. Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization[J]. Burns & Trauma, 2021,9(1). DOI： 10.1093/burnst/tkab008.

Zengjun Yang, Xiaohong Hu, Lina Zhou, Yaxiong He, Xiaorong Zhang, Jiacai Yang, Zhenyu Ju, Yih-Cherng Liou, Han-Ming Shen, Gaoxing Luo, Michael R Hamblin, Weifeng He, Rui Yin, Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization, Burns & Trauma, Volume 9, 2021, tkab008, https://doi.org/10.1093/burnst/tkab008 DOI：

摘要

Abstract

BackgroundEpidermal stem cells (EpSCs) that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis. Little is known about the effects of photochemical activation on EpSC differentiation

proliferation and migration during wound healing. The present study aimed to determine the effects of photodynamic therapy (PDT) on wound healing in vivo and in vitro.MethodsWe created mouse full-thickness skin resection models and applied 5-aminolevulinic acid (ALA) for PDT to the wound beds. Wound healing was analysed by gross evaluation and haematoxylin–eosin staining in vivo. In cultured EpSCs

protein expression was measured using flow cytometry and immunohistochemistry. Cell migration was examined using a scratch model; apoptosis and differentiation were measured using flow cytometry.ResultsPDT accelerated wound closure by enhancing EpSC differentiation

proliferation and migration

thereby promoting re-epithelialization and angiogenesis. PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines

whereas the secretion of growth factors was greater than in other groups. The proportion of transient amplifying cells was significantly greater in vivo and in vitro in the PDT groups. EpSC migration was markedly enhanced after ALA-induced PDT.ConclusionsTopical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization

promoting angiogenesis as well as modulating skin homeostasis. This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.

关键词

Keywords

Photodynamic therapyWound healing5-aminolevulinic acidre-epithelializationepidermal stem cellsTransient amplifying cells

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