Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis

Peng Hu; Anna Chiarini; Jun Wu; Giuliano Freddi; Kaiyu Nie; Ubaldo Armato; Ilaria Dal Prà

doi:10.1093/burnst/tkab003

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Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis

更新时间：2022-06-14

- Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis
- Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis
- Burns & Trauma 2021年9卷第1期
- 作者机构：
  
  1. Human Histology & Embryology Section
  2. University of Verona Medical School
  3. Department of Burns & Plastic Surgery, The Affiliated Hospital of ZunYi Medical University
  4. Department of Burns and Plastic Surgery
  5. University of Shenzhen
  6. Silk Biomaterials S..l,r
- 作者简介：
- 基金信息：
  
  The FUR 2018 of the Ministry of Italian University and Research funded this research work. No further funding from private or commercial sources supported it. PH holds a triannual doctorate student fellowship from the INVITE project funded by the European Union’s Horizon 2020 Research and Innovation Program under the Marie Sklodowska-Curie Grant Agreement No. 754345.
- DOI：10.1093/burnst/tkab003
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-5-4，
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Peng Hu, Anna Chiarini, Jun Wu, 等. Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis[J]. Burns & Trauma, 2021,9(1).

Peng Hu, Anna Chiarini, Jun Wu, Giuliano Freddi, Kaiyu Nie, Ubaldo Armato, Ilaria Dal Prà, Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis, Burns & Trauma, Volume 9, 2021, tkab003, https://doi.org/10.1093/burnst/tkab003
Peng Hu, Anna Chiarini, Jun Wu, 等. Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis[J]. Burns & Trauma, 2021,9(1). DOI： 10.1093/burnst/tkab003.

Peng Hu, Anna Chiarini, Jun Wu, Giuliano Freddi, Kaiyu Nie, Ubaldo Armato, Ilaria Dal Prà, Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis, Burns & Trauma, Volume 9, 2021, tkab003, https://doi.org/10.1093/burnst/tkab003 DOI：

摘要

Abstract

BackgroundBombyx mori silk fibroin is a biomacromolecule that allows the assembly of scaffolds for tissue engineering and regeneration purposes due to its cellular adhesiveness

high biocompatibility and low immunogenicity. Earlier work showed that two types of 3D silk fibroin nonwovens (3D-SFnws) implanted into mouse subcutaneous tissue were promptly vascularized via undefined molecular mechanisms. The present study used nontumorigenic adult human dermal fibroblasts (HDFs) adhering to a third type of 3D-SFnws to assess whether HDFs release exosomes whose contents promote neoangiogenesis.MethodsElectron microscopy imaging and physical tests defined the features of the novel carded/hydroentangled 3D-SFnws. HDFs were cultured on 3D-SFnws and polystyrene plates in an exosome-depleted medium. DNA amounts and D-glucose consumption revealed the growth and metabolic activities of HDFs on 3D-SFnws. CD9-expressing total exosome fractions were from conditioned media of 3D-SFnws and 2D polystyrene plates HDF cultures. Angiogenic growth factors (AGFs) in equal amounts of the two groups of exosomal proteins were analysed via double-antibody arrays. A tube formation assay using human dermal microvascular endothelial cells (HDMVECs) was used to evaluate the exosomes’ angiogenic power.ResultsThe novel features of the 3D-SFnws met the biomechanical requirements typical of human soft tissues. By experimental day 15

3D-SFnws-adhering HDFs had increased 4.5-fold in numbers and metabolized 5.4-fold more D-glucose than at day 3 in vitro. Compared to polystyrene-stuck HDFs

exosomes from 3D-SFnws-adhering HDFs carried significantly higher amounts of AGFs

such as interleukin (IL)-1α

IL-4 and IL-8; angiopoietin-1 and angiopoietin-2; angiopoietin-1 receptor (or Tie-2); growth-regulated oncogene (GRO)-α

GRO-β and GRO-γ; matrix metalloproteinase-1; tissue inhibitor metalloproteinase-1; and urokinase-type plasminogen activator surface receptor

but lesser amounts of anti-angiogenic tissue inhibitor metalloproteinase-2 and pro-inflammatory monocyte chemoattractant protein-1. At concentrations from 0.62 to 10 μg/ml

the exosomes from 3D-SFnws-cultured HDFs proved their angiogenic power by inducing HDMVECs to form significant amounts of tubes in vitro.ConclusionsThe structural and mechanical properties of carded/hydroentangled 3D-SFnws proved their suitability for tissue engineering and regeneration applications. Consistent with our hypothesis

3D-SFnws-adhering HDFs released exosomes carrying several AGFs that induced HDMVECs to promptly assemble vascular tubes in vitro. Hence

we posit that once implanted in vivo

the 3D-SFnws/HDFs interactions could promote the vascularization and repair of extended skin wounds due to burns or other noxious agents in human and veterinary clinical settings.

关键词

Keywords

Silk fibroinNonwovensDermisFibroblastHuman endothelial cellExosomeCytokineChemokineAngiogenesisRegenerationTissue engineering

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