In

Pseudomonas aeruginosa

(

P. aeruginosa

)

transcription factors (TFs) are important mediators in the genetic regulation of adaptability and pathogenicity to respond to multiple environmental stresses and host defences

.

The

P. aeruginosa

genome harbours 371 putative TFs; of these

about 70 have been shown to regulate virulence-associated phenotypes by binding to the promoters of their target genes. Over the past three decades

several techniques have been applied to identify TF binding sites on the

P. aeruginosa

genome

and an atlas of TF binding patterns has been mapped. The virulence-associated regulons of TFs show complex crosstalk in

P. aeruginosa

's regulatory network. In this review

we summarise the recent literature on TF regulatory networks involved in the quorum-sensing system

biofilm formation

pyocyanin synthesis

motility

the type III secretion system

the type VI secretion system

and oxidative stress responses

.

We discuss future perspectives that could provide insights and targets for preventing clinical infections caused by

P. aeruginosa

based on the global regulatory network of transcriptional regulators.