Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination

Xiaojian Cao; Xiaohua Chen; Yaqi Zhu; Xiaojuan Gou; Keyi Yan; Bing Yang; Dong Men; Lei Liu; Yong-an Zhang; Gang Cao

doi:10.1016/j.gendis.2022.08.020

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Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination

Full-length article | 更新时间：2023-08-29

- Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination
- Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination
- Genes & Diseases 2023年10卷第4期
- 作者机构：
  
  1. State Key Laboratory of Agricultural Microbiology, College of Fisheries, Huazhong Agricultural University,Hubei,Wuhan,China,430072
  2. State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences,Hubei,Wuhan,China,430071
  3. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University,Hubei,Wuhan,China,430072
  4. Spatial FISH Co. Ltd.,Hubei,Jiangmen,China,529199
  5. Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University,Guangdong,Guangzhou,China,51015
  6. Department of Transfusion Medicine, General Hospital of Central Theater Command,, Wuhan,Hubei,PLA,China,430070
  7. Department of Laboratory Medicine, Maternal and Child Health Hospital of Hubei Province,Hubei,Wuhan,China,430070
  8. Bio-Medical College, Huazhong Agricultural University,Hubei,Wuhan,China,430072
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.08.020
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-7-1，
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Xiaojian Cao, Xiaohua Chen, Yaqi Zhu, 等. Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination[J]. Genes & Diseases, 2023,10(4).

Xiaojian Cao, Xiaohua Chen, Yaqi Zhu, et al. Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination[J]. Genes & Diseases, 2023,10(4).
Xiaojian Cao, Xiaohua Chen, Yaqi Zhu, 等. Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination[J]. Genes & Diseases, 2023,10(4). DOI： 10.1016/j.gendis.2022.08.020.

Xiaojian Cao, Xiaohua Chen, Yaqi Zhu, et al. Single-cell transcriptome landscape and antigen receptor dynamic during SARS-CoV-2 vaccination[J]. Genes & Diseases, 2023,10(4). DOI： 10.1016/j.gendis.2022.08.020.

摘要

Abstract

Vaccination by inactivated vaccine is an effective strategy to prevent the COVID-19 pandemic. However

the detailed molecular immune response at single-cell level is poorly understood. In this study

we systematically delineated the landscape of the pre- and post-vaccination single-cell transcriptome

TCR (T cell antigen receptor) and BCR (B cell antigen receptor) expression profile of vaccinated candidates. The bulk TCR sequencing analysis of COVID-19 patients was also performed. Enrichment of a clonal CD8

T cell cluster expressing specific TCR was identified in both vaccination candidates and COVID-19 patients. These clonal CD8

T cells showed high expression of cytotoxicity

phagosome and antigen presentation related genes. The cell–cell interaction analysis revealed that monocytes and dendritic cells could interact with these cells and initiate phagocytosis via ICAM1-ITGAM and ITGB2 signaling. Together

our study systematically deciphered the detailed immunological response during SARS-CoV-2 vaccination and infection. It may facilitate understanding the immune response and the T-cell therapy against COVID-19.

关键词

Keywords

COVID-19SARS-Cov-2Single-cell sequencingTCR sequencingVaccination

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