JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA <em>MALAT1</em>

Junping Liu; Yutong Shi; Minmin Wu; Fengmei Zhang; Mengqi Xu; Zhiqiang He; Min Tang

doi:10.1016/j.gendis.2022.07.006

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JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1

Full-length article | 更新时间：2023-08-29

- JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1
- JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1
- Genes & Diseases 2023年10卷第5期
- 作者机构：
  
  Key Laboratory of Laboratory Medical Diagnostics Designated by the Chinese Ministry of Education, Chongqing Medical University,Chongqing,China,400016
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.07.006
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-9-1，
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Junping Liu, Yutong Shi, Minmin Wu, 等. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1[J]. Genes & Diseases, 2023,10(5).

Junping Liu, Yutong Shi, Minmin Wu, et al. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1[J]. Genes & Diseases, 2023,10(5).
Junping Liu, Yutong Shi, Minmin Wu, 等. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1[J]. Genes & Diseases, 2023,10(5). DOI： 10.1016/j.gendis.2022.07.006.

Junping Liu, Yutong Shi, Minmin Wu, et al. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1[J]. Genes & Diseases, 2023,10(5). DOI： 10.1016/j.gendis.2022.07.006.

摘要

Abstract

Despite significant improvements in five-year survival rates due to early diagnosis and combination therapy

triple-negative breast cancer (TNBC) treatment remains a major challenge. Finding new and effective targets for diagnosis and drug therapy is urgent for TNBC patients. Jagged-1 (JAG1)

one of the canonical ligands of the Notch signaling pathway

is involved in vascular budding and is a poor prognostic factor of TNBC. In this study

combined with quantitative real-time PCR

database analysis

animal experiments

and other means

JAG1 was confirmed to be related to the poor prognosis of TNBC patients.

JAG1

was highly expressed in MDA-MB-231 Bone (231B) cells

with stronger invasion and metastasis ability than MDA-MB-231 (231) cells. Treatment of human vascular endothelial cells (HUVEC) with TNBC conditioned medium showed that TNBC JAG1 promoted the angiogenesis of HUVEC. Next

we detected the exosomes extracted from TNBC conditioned medium and found that JAG1 promoted the exosome secretion from 231 cells via ALIX-RAB11A/RAB35. In addition

we also found that the exosomes from JAG1 overexpressed TNBC cells contained more long non-coding RNA (lncRNA)

MALAT1

and

MALAT1

promoted angiogenesis of HUVEC by targeting

miR-140-5p

. Finally

the angiogenesis-promoting effect of JAG1 in TNBC was further investigated by matrix gel assay. In conclusion

we reveal that JAG1 has a pro-invasion effect on TNBC and is involved in microenvironment angiogenesis by promoting exosome secretion and the MALAT1-miR-140-5p-JAG1/VEGFA pathway.

关键词

Keywords

AngiogenesisExosomeJAG1lncRNAMALAT1Triple-negative breast cancer

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JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1

JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1

DOI：10.1016/j.gendis.2022.07.006

摘要

Abstract

关键词

Keywords

references