Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis

Tingwei Zhang; Ziqi Chen; Mengyuan Zhu; Xuan Jing; Xiaohui Xu; Xulei Yuan; Mengjiao Zhou; Yanan Zhang; Miao Lu; Duanjing Chen; Shihan Xu; Jinlin Song

doi:10.1016/j.gendis.2022.06.009

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Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis

Full-length article | 更新时间：2023-08-29

- Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis
- Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis
- Genes & Diseases 2023年10卷第4期
- 作者机构：
  
  Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory for Oral Biomedical Engineering of Higher Education, and Stomatological Hospital of Chongqing Medical University,Chongqing,China,401147
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.06.009
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-7-1，
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Tingwei Zhang, Ziqi Chen, Mengyuan Zhu, 等. Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis[J]. Genes & Diseases, 2023,10(4).

Tingwei Zhang, Ziqi Chen, Mengyuan Zhu, et al. Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis[J]. Genes & Diseases, 2023,10(4).
Tingwei Zhang, Ziqi Chen, Mengyuan Zhu, 等. Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis[J]. Genes & Diseases, 2023,10(4). DOI： 10.1016/j.gendis.2022.06.009.

Tingwei Zhang, Ziqi Chen, Mengyuan Zhu, et al. Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis[J]. Genes & Diseases, 2023,10(4). DOI： 10.1016/j.gendis.2022.06.009.

摘要

Abstract

Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have emerged as a new mode of intercellular crosstalk and are responsible for many of the therapeutic effects of MSCs. To promote the application of MSC-EVs

recent studies have focused on the manipulation of MSCs to improve the production of EVs and EV-mediated activities. The current paper details an optimization method using non-invasive low-intensity pulsed ultrasound (LIPUS) as the stimulation for improving oral MSC-EV production and effectiveness. Stem cells from apical papilla (SCAP)

a type of oral mesenchymal stem cell

displayed intensity-dependent pro-osteogenic and anti-inflammatory responses to LIPUS without significant cytotoxicity or apoptosis. The stimuli increased the secretion of EVs by promoting the expression of neutral sphingomyelinases in SCAP. In addition

EVs from LIPUS-induced SCAP exhibited stronger efficacy in promoting the osteogenic differentiation and anti-inflammation of periodontal ligament cells

in vitro

and alleviating oral inflammatory bone loss

in vivo

. In addition

LIPUS stimulation affected the physical characteristics and miRNA cargo of SCAP-EVs. Further investigations indicated that miR-935 is an important mediator of the pro-osteogenic and anti-inflammatory capabilities of LIPUS-induced SCAP-EVs. Taken together

these findings demonstrate that LIPUS is a simple and effective physical method to optimize SCAP-EV production and efficacy.

关键词

Keywords

Extracellular vesiclesLow-intensity pulsed ultrasoundOral bone lossPeriodontitisStem cells from apical papilla

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