Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis

Yu Qiu; Yueyang Zhao; Zhimin Long; Aijia Song; Peng Huang; Kejian Wang; Ling Xu; David Paul Molloy; Guiqiong He

doi:10.1016/j.gendis.2021.06.008

您当前的位置：

首页 >

文章列表页 >

Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis

Full-length article | 更新时间：2023-08-29

- Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis
- Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis
- Genes & Diseases 2023年10卷第1期
- 作者机构：
  
  1. College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education,Chongqing,China,400016
  2. Chongqing Key Laboratory of Neurobiology, Chongqing Medical University,Chongqing,China,400016
  3. Department of Anatomy, Chongqing Medical University,Chongqing,China,400016
  4. Laboratory of Medical Experiment Technology, Institute of Life Science, Chongqing Medical University,Chongqing,China,400016
  5. Department of Biochemistry and Molecular Biology, Chongqing Medical University,Chongqing,China,400016
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2021.06.008
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-1-1，
扫描看全文
Yu Qiu, Yueyang Zhao, Zhimin Long, 等. Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis[J]. Genes & Diseases, 2023,10(1).

Yu Qiu, Yueyang Zhao, Zhimin Long, et al. Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis[J]. Genes & Diseases, 2023,10(1).
Yu Qiu, Yueyang Zhao, Zhimin Long, 等. Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis[J]. Genes & Diseases, 2023,10(1). DOI： 10.1016/j.gendis.2021.06.008.

Yu Qiu, Yueyang Zhao, Zhimin Long, et al. Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis[J]. Genes & Diseases, 2023,10(1). DOI： 10.1016/j.gendis.2021.06.008.

摘要

Abstract

Osteoporosis (OP) is a debilitating skeletal abnormality involving bone remodeling and bone cell homeostasis characterized by decreased bone strength and high fracture risk. A novel therapeutic intervention for OP by manipulating cellular autophagy–apoptosis processes to promote skeletal homeostasis is presented. Protective effects of the naturally occurring plant extract Liquiritigenin (LG) were demonstrated in an ovariectomy (OVX)-OP mouse model and preosteoblast MC3T3-E1 cells. Micro-CT and histological staining assessments of skeletal phenotype were applied alongside detection of autophagy activity in osteocytes and MC3T3-E1 cells by transmission electron microscopy (TEM). The effects of LG on chloroquine (CQ)- and the apoptosis-inducing TS-treated osteogenic differentiations and status of lysosomes within MC3T3-E1 cells were analyzed by Neutral red

Alizarin red S and alkaline phosphatase (ALP) staining and Western blot assays. Treatment with LG prevented bone loss

increased osteogenic differentiation

in vivo

and

in vitro

and inhibited osteoclast formation to some extent. TEM analyses revealed that LG can improve auto-lysosomal degradation within osteocytes from OVX mice and MC3T3-E1 cells. The abnormal status of lysosomes associated with CQ and TS treatments was notably alleviated by LG which also reduced levels of apoptosis-induced inhibition of osteogenic differentiation and averted abnormal osteogenic differentiation as a consequence of a blockage in autolysosome degradation. Overall

LG stimulates bone growth in OVX mice through increased osteogenic differentiation and regulation of autophagy-apoptosis mechanisms

presenting an auspicious natural therapy for OP.

关键词

Keywords

ApoptosisAuto-lysosomal degradationLiquiritigeninOsteogenic differentiationOsteoporosis

references

查看原文

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

BMP9 induces osteogenic differentiation through up-regulating LGR4 via the mTORC1/Stat3 pathway in mesenchymal stem cells

Autophagy-related lncRNAs in tumor progression and drug resistance: A double-edged sword

Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway

Molecular and cellular pathophysiology of circulating cardiomyocyte-specific cell free DNA (cfDNA): Biomarkers of heart failure and potential therapeutic targets

PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis