PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling

Sicheng Chen; Mengjun Bie; Xiaowen Wang; Mengtian Fan; Bin Chen; Qiong Shi; Yingjiu Jiang

doi:10.1016/j.gendis.2021.05.005

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PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling

Full-length article | 更新时间：2022-11-09

- PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling
- PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling
- Genes & Diseases 2022年9卷第6期
- 作者机构：
  
  1. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University,Chongqing,PR China,400016
  2. Ministry of Education Key Laboratory of Diagnostic Medicine, School of Laboratory Medicine, Chongqing Medical University,Chongqing,PR China,400016
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2021.05.005
  中图分类号：
- 纸质出版日期：2022，
  
  网络出版日期：2022-11-1，
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PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling[J]. Genes & Diseases, 2022,9(6).

Sicheng Chen, Mengjun Bie, Xiaowen Wang, et al. PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling[J]. Genes & Diseases, 2022,9(6).
PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling[J]. Genes & Diseases, 2022,9(6). DOI： 10.1016/j.gendis.2021.05.005.

Sicheng Chen, Mengjun Bie, Xiaowen Wang, et al. PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling[J]. Genes & Diseases, 2022,9(6). DOI： 10.1016/j.gendis.2021.05.005.

摘要

Abstract

Progranulin (PGRN) is a growth factor that is involved in the progression of multiple tumors. However

the effects and molecular mechanisms by which PGRN induces lung cancer remain unclear. The expression level of PGRN was analyzed by conducting immunohistochemistry of the histological sections of lung tissues from non-small-cell lung carcinoma (NSCLC) patients. The proliferation

apoptosis

migration

and invasion of NSCLC cells were assessed by the MTT assay

Western blot

degree of wound healing

and Transwell assays. A nude mouse xenograft model was used to validate the role of PGRN

in vivo

. The expression level of PGRN was higher in male patients with lung adenocarcinoma than in those with lung squamous cell carcinoma; by contrast

no difference was observed in female patients. The overexpression of PGRN promoted the proliferation and anti-apoptosis of H520 (derived from lung squamous cell carcinoma) cells

whereas knockdown of PGRN inhibited the proliferation and anti-apoptosis of A549 (derived from lung adenocarcinoma) cells. Copanlisib (targeting PI3K) inhibited the increase in the expression of cell anti-apoptosis marker Bcl-2 induced by rhPGRN protein; the PI3K agonist 740 Y–P partially reversed the decrease in Bcl-2 expression induced by PGRN deficiency in both A549 and H520 cells. PGRN increased the expression of Ki-67

PCNA

and Bcl-2

in vivo

. PGRN inhibited cell apoptosis depending on the PI3K/Akt/Bcl-2 signaling axis; PGRN positivity correlated with lung adenocarcinoma. PGRN is a potential biomarker for the treatment and diagnosis of NSCLC

especially in lung adenocarcinoma.

关键词

Keywords

Bcl-2Cell apoptosisNSCLCPGRNPI3K/Akt

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