A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples

Yong-guo Zhang; Yinglin Xia; Jun Sun

doi:10.1016/j.gendis.2020.03.002

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A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples

Short communication | 更新时间：2022-06-24

- A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples
- A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples
- Genes & Diseases 2021年8卷第6期
- 作者机构：
  
  1. Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago,IL,Chicago,USA,60612
  2. UIC Cancer Center, University of Illinois at Chicago,IL,Chicago,USA,60612
  3. Department of Microbiology and Immunology, University of Illinois at Chicago,IL,Chicago,USA,60612
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2020.03.002
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-11-1，
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Yong-guo Zhang, Yinglin Xia, Jun Sun. A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples[J]. Genes & Diseases, 2021,8(6).

Yong-guo Zhang, Yinglin Xia, Jun Sun. A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples[J]. Genes & Diseases, 2021,8(6).
Yong-guo Zhang, Yinglin Xia, Jun Sun. A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples[J]. Genes & Diseases, 2021,8(6). DOI： 10.1016/j.gendis.2020.03.002.

Yong-guo Zhang, Yinglin Xia, Jun Sun. A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples[J]. Genes & Diseases, 2021,8(6). DOI： 10.1016/j.gendis.2020.03.002.

摘要

Abstract

Vitamin D receptor (VDR) executes the main biological functions of its ligand vitamin D. VDR/vitamin D plays critical roles in regulating host immunity

maintaining barrier functions

and shaping gut microbiome. Reduction of intestinal VDR has been reported in various diseases

including inflammatory diseases and colon cancer. However

it is always challenging to get biopsies to test the pathologic changes of VDR in intestine. In the current study

we reported a simple and sensitive quantitative PCR (qPCR) method to detect reduction of intestinal VDR using fecal samples. We validated this method in several experimental models

such as colitis

bacterial infection

and aging. We further correlated the qPCR data of VDR with the protein level of VDR in colon or serum 25 (OH)D

in mice with different VDR status (VDR

+/+

VDR

+/-

and VDR

−/−

). Our data indicate that the qPCR method to test VDR using fecal samples could detect the expression level of intestinal VDR in various diseases. Our study highlights the feasibility

sensitivity

and simplicity of a molecular method to study the status of VDR as a biomarker.

关键词

Keywords

AgingBiomarkerCorrelationInfectionInflammationMicrobiomeSalmonellaVitamin D deficiency

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