Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells

Xiaogen Ma; Fei Xiang; Zhuo Pei; Jiafeng Miao; Pan Wu; Xiaofeng Song; Yuhong Li; Yiming Zhang

doi:10.1016/j.gendis.2020.01.001

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Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells

Full-length article | 更新时间：2022-06-24

- Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells
- Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells
- Genes & Diseases 2021年8卷第3期
- 作者机构：
  
  1. Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Army Medical University,Chongqing,PR China,400037
  2. Department of Cell Biology, Army Medical University,Chongqing,PR China,400038
  3. Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Army Medical University,Chongqing,PR China,400038
  4. Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics,Nanjing,PR China,211106
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2020.01.001
  中图分类号：
- 纸质出版日期：2021，
  
  网络出版日期：2021-5-1，
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Xiaogen Ma, Fei Xiang, Zhuo Pei, 等. Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells[J]. Genes & Diseases, 2021,8(3).

Xiaogen Ma, Fei Xiang, Zhuo Pei, et al. Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells[J]. Genes & Diseases, 2021,8(3).
Xiaogen Ma, Fei Xiang, Zhuo Pei, 等. Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells[J]. Genes & Diseases, 2021,8(3). DOI： 10.1016/j.gendis.2020.01.001.

Xiaogen Ma, Fei Xiang, Zhuo Pei, et al. Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells[J]. Genes & Diseases, 2021,8(3). DOI： 10.1016/j.gendis.2020.01.001.

摘要

Abstract

Circular RNAs are a large class of noncoding RNAs. Smad5 functions in cell differentiation

cell proliferation and metastasis. It has been reported that lnc-Smad5 can inhibit the proliferation of diffuse large B cell lymphoma. However

the function of circ-Smad5 has not yet been reported. Lentivirus vectors were constructed to establish circ-Smad5 upregulated and circ-Smad5 downregulated cell models. A CCK-8 assay was used to detect the proliferation of JB6 cells. FACS was used to analyze the cell cycle in the cell models. Western blot

immunofluorescence staining and TOP/FOP flash dual luciferase activity assays were used to determine the activity of the Wnt signaling pathway. The results revealed that the expression level of circ-Smad5 in JB6 cells was significantly lower than the expression level of linearized-Smad5. Compared with the control group

the percentage of S phase cells and the expression level of cyclin D1 protein were significantly higher in the sh-circ-Smad5 group. In the sh-circ-Smad5 group

β-catenin and LEF-1 were significantly increased

p-β-catenin was significantly decreased

and the relative activity of the TOP/FOP reporter gene was higher compared to the control group levels. These phenomena could be reversed by treating with Wnt signaling inhibitor PNU-74654. We conclude that the circ-Smad5 retards the proliferation and the cell cycle progression of JB6 cells. Thus

circ-Smad5 may function by inhibiting the activation of Wnt/β-catenin/Lef 1 signaling

which inhibits the expression of cyclin D1. To the best of our knowledge

we are the first to report the function of circ-Smad5.

关键词

Keywords

Cell cycleCirc-Smad5Circular RNAWnt signalingCell proliferation

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