Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells

Bo Huang; Lin-Feng Huang; Ling Zhao; Zongyue Zeng; Xi Wang; Daigui Cao; Lijuan Yang; Zhenyu Ye; Xian Chen; Bin Liu; Tong-Chuan He; Xiaozhong Wang

doi:10.1016/j.gendis.2019.04.005

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Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells

Full-length article | 更新时间：2022-06-24

- Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells
- Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells
- Genes & Diseases 2020年7卷第2期
- 作者机构：
  
  1. Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory Medicine, and The Affiliated Hospitals of Chongqing Medical University, Chongqing Medical University,Chongqing,China,400016
  2. Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center,IL,Chicago,USA,60637
  3. Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Nanchang University,Nanchang,China,330006
  4. Department of Clinical Laboratory Medicine, Jiangxi Maternal and Child Health Hospital,Nanchang,China,330006
  5. Department of Orthopaedic Surgery, Chongqing General Hospital Affiliated with the University of Chinese Academy of Sciences,Chongqing,China,400013
  6. Department of Obstetrics and Gynecology, The First Hospital of Lanzhou University,Lanzhou,China,730030
  7. Department of General Surgery, The Second Affiliated Hospital of Soochow University,Suzhou,China,215004
  8. Department of Clinical Laboratory Medicine, The Affiliated Hospital of Qingdao University,Qingdao,China,266061
  9. Department of Biology, School of Life Sciences, Southwest University,Chongqing,China,400715
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2019.04.005
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-6-1，
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Bo Huang, Lin-Feng Huang, Ling Zhao, 等. Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells[J]. Genes & Diseases, 2020,7(2).

Bo Huang, Lin-Feng Huang, Ling Zhao, et al. Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells[J]. Genes & Diseases, 2020,7(2).
Bo Huang, Lin-Feng Huang, Ling Zhao, 等. Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells[J]. Genes & Diseases, 2020,7(2). DOI： 10.1016/j.gendis.2019.04.005.

Bo Huang, Lin-Feng Huang, Ling Zhao, et al. Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells[J]. Genes & Diseases, 2020,7(2). DOI： 10.1016/j.gendis.2019.04.005.

摘要

Abstract

Extracellular vesicles (EVs) such as microvesicles (MIVs) play an important role in intercellular communications. MIVs are small membrane vesicles sized 100–1000 nm in diameter that are released by many types of cells

such as mesenchymal stem cells (MSCs)

tumor cells and adipose-derived stem cells (ADSC). As EVs can carry out autocrine and paracrine functions by controlling multiple cell processes

it is conceivable that EVs can be used as delivery vehicles for treating several clinical conditions

such as to improve cardiac angiogenesis after myocardial infarction (MI). Here

we seek to investigate whether ADSC-derived MIVs contain microRNAs that regulate angiogenesis and affect cell migration of endothelial cells. We first characterized the ADSC-derived MIVs and found that the MIVs had a size range of 100–300 nm

and expressed the MIV marker protein Alix. We then analyzed the microRNAs in ADSCs and ADSC-derived MIVs and demonstrated that ADSC-derived MIVs selectively released a panel of microRNAs

several of which were related to angiogenesis

including two members of the let-7 family. Furthermore

we demonstrated that ADSC-derived MIVs promoted the cell migration and invasion of the HUVEC endothelial cells. The PKH26-labeled ADSC-derived MIVs were effectively uptaken into the cytoplasm of HUVEC cells. Collectively

our results demonstrate that the ADSC-derived MIVs can promote migration and invasion abilities of endothelial cells

suggesting pro-angiogenetic potential. Future studies should focus on investigating the roles and mechanisms through which ADSC-derived MIVs regulate angiogenesis.

关键词

Keywords

Adipose-derived stem cells (ADSCs)AngiogenesisLet-7microRNAMicrovesicle (MIV)

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