Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq

Jin Xiang; Yuhong Zhang; Lin Tuo; Rui Liu; Dongmei Gou; Li Liang; Chang Chen; Jie Xia; Ni Tang; Kai Wang

doi:10.1016/j.gendis.2019.04.004

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Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq

Full-length article | 更新时间：2022-06-24

- Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq
- Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq
- Genes & Diseases 2020年7卷第1期
- 作者机构：
  
  1. Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University,Chongqing,China,400010
  2. The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University,Chongqing,China,400010
  3. Institute of Life Sciences, Chongqing Medical University,Chongqing,China,400010
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2019.04.004
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-3-1，
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Jin Xiang, Yuhong Zhang, Lin Tuo, 等. Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq[J]. Genes & Diseases, 2020,7(1).

Jin Xiang, Yuhong Zhang, Lin Tuo, et al. Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq[J]. Genes & Diseases, 2020,7(1).
Jin Xiang, Yuhong Zhang, Lin Tuo, 等. Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq[J]. Genes & Diseases, 2020,7(1). DOI： 10.1016/j.gendis.2019.04.004.

Jin Xiang, Yuhong Zhang, Lin Tuo, et al. Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq[J]. Genes & Diseases, 2020,7(1). DOI： 10.1016/j.gendis.2019.04.004.

摘要

Abstract

Phosphoenolpyruvate carboxykinase 1 (PCK1)

a step limiting enzyme of gluconeogenesis

is downregulated in hepatocellular carcinoma (HCC). Overexpression of PCK1 has been shown to suppress hepatoma cell growth

but the underlying mechanism remains unclear. We used recombinant adenovirus overexpressing PCK1 or GFP in Huh7 cells

and the differentially expressed genes (DEGs) were identified by RNA-Seq. 180 were upregulated by PCK1 overexpression

whereas 316 were downregulated. Pathway analysis illustrated that PCK1 was closely correlated with Wnt signaling pathway and TGF-beta signaling pathway. Hence

Wnt signaling pathway and its downstream component

FZD2

FZD6

FZD7 and β-catenin were confirmed by qRT-PCR and Western blot. In vivo we also observed that PCK1 had restrained tumor growth as a result of decreasing expression of β-catenin. Whole-transcriptomic profile analysis discovered that overexpression of PCK1 downregulates several oncogenic signaling pathways in HCC

providing potential therapeutic targets for improving HCC therapy.

关键词

Keywords

Hepatocellular carcinoma cellsOncogenePhosphoenolpyruvate carboxylase kinase1 (PCK1)RNA sequencingWnt signaling pathway

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