Objective　On the background of the clinical skills competition for national medi-cal students in the higher medical colleges and universities

to investigate medical students' mastery of hand hygiene knowledge and skills

explore the effective methods of teaching

and improve the medical students' hand hygiene compliance and accuracy in clinical practice. Methods　90 medical students at a tertiary-level teaching hospital clinical practice stage were randomly selected for the study

and performed hand hygiene theoretical knowledge and practical skills training in special education. Special teaching investigation was made before and after training

to investigate the students' hand hygiene compliance rate in clinical practice. And the correct rate of hand hygiene were examined and medical students' hand hygiene compliance rate and correct rate were compared before and after the special teaching and training. Statistical analysis was made by using chi square test 2. Results　After theory teaching and additional special skills training the students' hand hygiene compliance and accuracy were improved significantly (79.00% vs. 35.50%

P=0.000). The correct rate of hand hygiene was also significantly higher than before training (92.22% vs. 61.11%

P=0.000). Conclusion　Hand hygiene knowledge and skills training can significantly improve the medical students' hand hygiene compliance and accuracy in the clinical practice

and is conducive to the prevention of the spread of infection in hospitals

and then reduce the incidence of hospital infection.