【】 Optogenetics is one of the biggest breakthroughs in neurobiology in recent decades that uses a combination of techniques from genetic and optics to control and monitor the activities of individual neurons in living tissue—even within freely-moving animals—and to precisely measure these manipulation effects in real-time. However

the technique involves the interdisciplinary theoretical knowledge and methods

and it needs expensive equipments and long experiment period. Thus

it is not convenient to carry out widely in experiment teaching for undergraduates except the virtual simulation teaching. In present virtual simulation experiment

we learn the basic principle

operation and application of optogenetics and experimental teaching get the desired results by simulating the process of the virus package

simulating the process of the animal selection

surgery and virus stereotactic microinjection

simulating the expression process of photosensitive channel

the extracellular verification process of photosensitive channel function in vivo

and simulating the observation process that optogenetic inhibition of glutamate neurons in the amygdala affect animal fear behavior. This paper introduced the content design of the script making of the virtual experiment.