Objective　To investigate the application effect of the method based on peer-assisted learning (PAL) in venipuncture training. Methods　A total of 120 new nurses who worked in Department of Neurology from May 2016 to January 2018 were enrolled and randomly divided into PAL group and control group. The nurses in the PAL group received venipuncture training with the PAL teaching mode

and those in the control group received venipuncture training with the conventional method. Practical operation ability was assessed after one month of training

including the ability to perform venipuncture

communication ability

humanistic care ability

and patient’s degree of satisfaction

and a questionnaire survey was conducted to evaluate teaching methods and teaching effect. SPSS 20.0 was used for statistical analysis. Results　There were significant differences between the PAL group and the control group in the scores of the ability to perform venipuncture (64.9±3.7 vs 62.8±4.7)

communication ability (8.6±1.3 vs 8.0±1.4)

humanistic care ability (8.8±1.2 vs 8.2±1.1)

and patient’s degree of satisfaction (8.8±1.2 vs 8.3±1.2). The questionnaire survey showed that the new nurses were more satisfied with the PAL training method

and the PAL group had significantly better learning efficiency

learning enthusiasm

and problem-solving ability than the control group (P<0.05). Conclusion　The PAL teaching method can improve the practical operation ability of new nurses and help to improve training efficiency and quality

and therefore

it can be used as an important teaching method for venipuncture training for new nurses.