To investigate the application of seminar teaching on postgraduate education of ophthal-mology, postgraduate from Grade 2008 to Grade 2010 were divided into experiment groups and control groups. Seminar teaching and conventional teaching were applied to two groups respectively. The perfor-mances of seminar teaching groups at each grade were better than the control groups(P<0.05). Thus, seminar teaching can enhance theory and operation ability of postgraduate.