Objective　To investigate effects of new nursing experimental teaching mode on professional ability of nursing students. Methods　Traditional experimental teaching mode was used in control group （402 nursing students of 2006 grade） and experimental teaching model was used in experimental group （564 nursing students of 2007 grade）. Questionnaires survey was conducted among clinical teachers to evaluate 8 abilities （service consciousness， knowledge application， practical abil-ity， communication ability. etc） of nursing students after 3 months' clinical practice. Satisfaction de-gree of experimental teaching and the first time employment rate of nursing students were recorded. All data were sorted out and analyzed by Epidata 3.1 and SPSS 13.0. Total score of students' profes-sional capacity was compared by independent t test. Score of the scale item was compared by rank sum test. Satisfaction rating for experimental teaching， passing rate of nurse practicing qualification examination and the first time employment rate were compared by chi-square test. All statistical tests were two-sided and P <0.05 was considered statistically significant different. Results　Students' scores of communication ability， innovation capacity， capability to identify problems， practical ability， knowl-edge application， ability of organization and independent learning ability were 2.86±0.23， 2.86±0.17， 2.85±0.19， 2.84±0.24， 2.79±0.20， 2.78±0.19， 2.65±0.19 in experimental group， higher than those in control group. Satisfaction degree of experimental teaching and the first employment rate of students in experimental group were 94.5%（536/567） and 84.5%（479/567）， higher than those in con-trol group with statistically significant differences（P<0.05）. Conclusion　New teaching mode of nurs-ing experiment can improve the professional ability of nursing students.