Objective　To discuss the effect of team-based learning（TBL） combined with net-work environment in pathological experiment teaching. Methods　Totally 112 clinical medical under-graduates were selected as research object. Digital microscope interactive teaching platform under the network environment was used in experimental group（n=56）. In pathology experiment teaching， TBL steps of training， teaching plan demonstrating， grouping， teaching task arranging， asking questions， group learning and communicating were implemented. Traditional method （lecture） was used in con-trol group （n=56）. Teaching effect was analyzed through questionnaire survey and score analysis. Stu-dents' evaluation on TBL teaching and network environment resources was expressed as percentage. Results　In experiment group， average score was （85.5±3.11） points and average final exam score was （83.8±2.53） points， while in control group， average final exam score was （76.4±11.89） points. There were significance differences between the two groups （t=7.018， P<0.01）. Surveys showed that most students accepted TBL teaching combined with network environment. Conclusions　TBL teach-ing combined with network environment is feasible and effective in the pathology experimental class. Students' learning enthusiasm is generally improved and effectiveness of TBL teaching is satisfactory.