Objective To investigate the effect of the evaluation on teaching hospitals affiliat-ed to medical colleges in Guangdong province. Methods Questionnaire and on on-the-spot investi-gation was conducted among 18 teaching hospitals which owned the accreditation for 2-3 years based on Guangdong provincial teaching hospital accreditation indicator system 2008 modified version. Contents of investigation included general situation, teaching condition, management and implementa-tion of teaching activities as well as medical staffs' comments and suggestions for the teaching work. SPSS 13.0 software was used for frequency analysis. Results The general situation,teaching condi-tion,management and implementation of teaching activities were improved to varying degrees after the evaluation according to results of the questionnaire. 90.0%(54/90) people thought that the overall scale of the hospital was expanded and 5.0%(3/60) people thought that the overall scale of the hos-pital was obviously reduced or marinated the original level. 11.6%(7/60) and 88.3%(53/60) people thought that medical quality of the hospital was improved or obviously improved. 60.0%(36/60) people thought that educational occupancy of the hospital was improved while 5.0%(3/60) people thought that it was unsatisfactory. 83.3%(50/60) people thought that teaching mold and infrastructures were improved while 10.0%(6/60) people thought that it was unsatisfactory. 95.0%(57/60) people thought that teaching management was improved. Due to the lack of incentives and support in teaching work, management of teaching was loose. Conclusions The teaching work of t hospitals affiliated to medical colleges in Guangdong province do persevere basically and achieve desired purposes.