Objective　To explore the effect of problem-based learning （PBL） in biochemistry teaching in medical college. Methods　Totally 128 students （2011 grade） of medical laboratory major were derived into experiment group （n=64） and control group （n=64）. Lecture-based learning （LBL） was used in control group and PBL was used in experiment group.In control group， teachers did teaching design according to the needs of curriculum standards， employed multimedia courseware， explained the basic content of each chapter， class exercises and homework after class. In experimental group， PBL was conducted through establishing biochemistry teaching website based on school's teaching platform. Teach-ing process in experiment group included： grouping-selecting appropriate cases-consulting data-group discussing-summarizing by teachers. Theory test was carried out after the course and questionnaire was conducted for students in experiment group. Data were analyzed using the independent sample t test. Results　Average test scores were higher in PBL group than in traditional group， with statistically signif-icant differences[（83.15±11.87） vs. （76.15±12.48）]. Questionnaire survey showed that more than 90.0% students in experiment group were interested in the PBL teaching method and thought that the PBL teach-ing method can enhance the enthu-siasm of learning， independent learning ability and comprehensive analysis ability. Conclusions　PBL teaching method can improve the quality and the effect of biochemi-stry teaching and culture students' comprehensive ability. It is worthy of further study and popularization.