Objective　To explore the application of students' evaluations of educational quality questionnaire（SEEQ） and related influencing factors in the teaching effectiveness evaluation of medical faculty. Methods　477 Grade 1-4 five-years medical students in China medical university were investi-gated to evaluate the teaching effectiveness. We used the internal consistency and principal component method to test the reliability and validity of the scales. We analyzed different influencing factors of educational quality including different gender， grade， student academic achievement and curriculum type with the t-test and AVONA in the SPSS 19.0 software， P<0.05 was considered statisti-cally. Results　The Cronbach's α coefficient of SEEQ was 0.89， the cumulative variance contribution rate was 86.43%， the reliability and validity of the questionnaire were good. Girls scored higher in the teacher-student relationships domain than boys（P=0.022）. Students in the lower grade had lower scores in the dimen-sions of the scales（P<0.05）. Clinical courses scored higher in all dimensions of the scales（P<0.05） than basic medical courses. Outstanding students had higher scores in the sense of the learning value， teacher-student relationships， the scope of teacher's knowledge and curriculum management than nor-mal students（P<0.05）. Conclusions　It is feasible that SEEQ questionnaire is applied in the teaching evaluation of medical faculty. Medical faculties should carry out teaching in－novation according to the characteristics of different students and course type with the targeted method.