Animal surgery curriculum is one of the important courses which lead the medical students from basic theory to clinical practice. It helps students to develop a good foundation for the later clinical work. Applications of collective lesson preparation together with second preparation in animal surgery curriculum can unify the animal surgery teaching standard， improve teachers' profes-sional quality and teaching ability， mobilize the teaching team's cooperation consciousness and ensure the overall level of the medical college animal surgery teaching when highlighting the personal teach-ing characteristics. This combination of lesson preparation and second preparation is a good teaching and research activity and worth popularizing.