Animal experiment is a primary part of medical education. At present， medical students are lack of education about laboratory animal ethics and they do not usually treat animals fol-lowing rules of laboratory animal ethics. To solve this problem， we focused on the education of labora-tory animal ethics and 3R theory （reduction， replacement， refinement） in order to help the medical students treat laboratory animals nicely. We enforced the construction of the laboratory rules and regu-lations， and improved animal experiments conditions. With these efforts， we successfully standardized and scientized our experiment classes.