Occupational health and occupational medicine is a main course of preventive medicine. Problem-based learning（PBL）was practiced in partial chapters of occupational health and occupational medicine course aiming at improving student's studying interest. Problems with certain degrees of openness and difficulty were selected， and students were organized to review literature， discuss in groups and report in class. The students responded in the feedback that PBL improved their comprehensive ability in discovering problem， reviewing literature， analyzing problem and reaching the conclusion. Basing on the problems in the practice of PBL， more detailed and impartial standard should be established in evaluating student's mark and the right direction should be held throughout PBL.