[Abstract] Objective To explore the effect of precision medicine-based comprehensive experimental teaching in the course of molecular biology experiment. Methods Eight-year medical students of Grade 2012 and 2013 in the Fourth Military Medical University were chosen as research objects. Experimental group consisted of 36 students of grade 2013

while control group consisted of 30 students of grade 2012. Precision medicine-based learning was applied in experimental group while traditional learning method was adopted by the control group. At the end of the course

the score of theory and operating skills of two groups was collected and students were investigated by providing individual question paper. The significance of the data was determined by Student's t test and Chi-square analysis using SPSS software. Results The assessment results showed that the experimental group was better than control group

especially in the section of comprehensive experimental design (16.7?2.04 vs. 13.9?2.87

P<0.0001). The results from questionnaire showed that the satisfaction degree of experimental group was also higher than that of control group in many respects

including learning interests

innovation capability

knowledge mastery

cognition of precision medicine and clinical research

satisfaction with the teaching method (P<0.05). Conclusions Precision medicine-based learning not only changes the situation of slavish imitation and passive acceptance in traditional learning

but also arouses student’s interest in study and helps students to cultivate clinical thinking which is commensurate to the characteristics of precision-medicine era.