Objective To discuss the application of micro assistant teaching in physiology teaching. Methods A total of 112 students from the rehabilitation class 1 and 2 of grade 2016 in Hubei University of Medicine were selected as the subjects. The class 1 was the control group and class 2 was the experimental group. The control group was adopted the traditional teaching method. The experimental group was adopted micro assistant teaching method for physiology teaching. The effect of micro assistant teaching in physiology teaching was evaluated by final examination and questionnaire. Results Statistical difference was found in average score between experiment group and control group (81.46±7.63 vs. 68.21±10.27) (P=0.000). Questionnaire survey of teaching effect showed that in experimental group 56 students (98.24%) felt it could help to enhance interactive communication

51 students (89.47%) believed it could help to organize the classroom discipline

43 students (75.43%) believed it could increase learning interest

and 55 students (95.26%) believed it could help to accurately solve the various difficult points of knowledge. Conclusion Micro assistant teaching can improve the teaching effect of physiology teaching

which is worthy of promotion.