Objective　To investigate the value of 3D printing technology practical training applied in practical teaching of graduate students of otorhinolaryngology. Methods　Total 42 graduate students of otorhinolaryngology were divided into control group (20) and experimental group (22) randomly. The control group received traditional teaching mode while the experimental group received 3D printing technology practical training mode. The effect of teaching modes was evaluated by questionnaire and theoretical and practical examinations. The data were analyzed through t test of SPSS 17.0. Result　Questionnaire survey of the different teaching modes showed that: the average score of control group was (59.4±3.5)

while that of experimental group was (64.6±3.6). Theoretical examination score showed that: the average score of control group was (75.7±5.1)

while that of experimental group was (81.5±7.7). Practical examination score showed that: the average score of control group was (74.5±5.8)

while that of experimental group was (80.9±6.9). All the differences are statistically significant (P<0.05). Conclusion　The recognition degree of 3D printing technology practical training in these graduate students was significantly higher than that of the traditional teaching mode

and the teaching effect from 3D printing technology practical training was better compared with that from the traditional teaching mode.