Objective　To investigate the effect of virtual-simulation laparoscopic training system in the training of basic skills of endoscopic surgery in trainees with different clinical experience. Methods Eight refresher physicians

eight residents who received standardized residency training

and eight undergraduate interns in five-year clinical medicine were selected. All of them received the training of endoscopic operations with the laparoscopic training box and the virtual-simulation laparoscopic training system for 30 minutes every day for 4 weeks. Data analyses were performed using SPSS 19.0 and t-test was adopted to compare the scores before and after training among the three groups. Results　Before training

there were significant differences in endoscopic operations between the undergraduate intern group and the other two groups (P<0.05); after 4 weeks of training

all three groups had significant increases in the scores and spent less time on training items (P<0.05). There was no significant difference in simple operations among the three groups (P>0.05)

and the undergraduate intern group had a significantly higher score of complex operations than did the standardized residency training group and the refresher physician group (P<0.05). Conclusion　The virtual-simulation laparoscopic training system can improve the laparoscopic skills of clinical trainees at different levels

therefore

it is worth being promoted in the teaching of clinical skills.