Objective　To explore the application effect of online-offline mixed teaching mode in the teaching of histology and embryology. Methods　Two classes of students majoring in psychiatry from Batch 2020 were randomized into experimental group (n = 100)

and two classes of students majoring in general practice in 2020 grade were randomized into the control group (n = 99). The online-offline mixed teaching mode was used in the experimental group

while a traditional teaching mode was used in the control group. The online test scores

final scores and questionnaire satisfaction of the two groups of students were evaluated on the teaching effect. All data were statistically processed by SPSS 19.0

and the comparison between groups was performed by t test. Results　The online test scores of the experimental group [(8.55±1.18) vs. (6.33±0.91)] and final scores [(85.56±3.32) vs. (72.24±2.71)] were significantly higher than those of the control group (P < 0.05). Compared with the control group

the experimental group's satisfaction scores of the online-offline mixed teaching mode were significantly higher in students' learning interest

learning autonomy

time management ability

teamwork

interactive communication

knowledge understanding and memory ability (P < 0.05). Conclusion　The online-offline mixed teaching mode can improve the histology and embryology teaching effect and promote the students' interest in learning and autonomous learning ability.