Yu Mingan, et al. A NEW TECHNIOUE FOR THE ISOLATION AND PURIFICATION OF HIGH PURITY FRUCTOSE-1,6-DIPHOSPHATE[J]. Journal of Chongqing Medical University, 1995,(1).
Yu Mingan, et al. A NEW TECHNIOUE FOR THE ISOLATION AND PURIFICATION OF HIGH PURITY FRUCTOSE-1,6-DIPHOSPHATE[J]. Journal of Chongqing Medical University, 1995,(1).DOI：
new technique for isolation and purification of fructose- 1
6- diphosphate（FDP）from the fermentation mixture has been reported
Which utilizes precipitation
absorptionand recrystallization instead of the reverse osmosis system or Chromatographic oncationic and anionic resins
molecular fiItration and lyophilization
Thus
4L of a fermen-tion anionic was deproteinized and precipItated usifig 50%（W/W）CaCl
2
Solution;FDPwas purified by ion exchange
microencapsulated charcoal treatment and recrystallization.The overall yield of FDP was 80%and the purity was 99%. The new technique short-ened the production period and was easy to scale up for industrial application.
关键词
1，6一二磷酸果糖分离精制重结晶
Keywords
Fructose-16-diphosphateIsolation and purificationRecrystallization
京公网安备11010802024621