he experunental conditions of 45Ca uptake into synaptosome from the mice cerebral corticeswere studied.It was found that during the firot 4 minutes there was a straight relationshipbetween amount of 45Ca uptake and incubation tune. The range of suitable membrane protein ofsynaptosame used was between 0.063～0.250 mg and 45Ca uptake would decline when membraneprotein was more than 0.250mg Low calcium medium(0.6mmol/L)was more beneficial to the 45Cauptake than zero or normal calcium(12mmol/L)medium

and nitrendipine had a promoting effecton 45Ca uptake in the former medium. The rang of kalium concentrations for the best stunulationof 45Ca uptake was grom 30 to 50 mmol/L. DMSO had a potential effect on 45Ca uptake at theconcentration of 0.05%

but ethanol and acetone failed to show an influence on it at theconcentrations of 0.05～6.25%。All results above provide essential data for correctly carrying outexperunents of 45Ca uptake into synaptcoolne and determining the effects of drugs on 45Ca uptake.