Zhang Yuhong, et al. Processed pseudogenes interfere with the reliability of reverse tran-scriptase - polymerase chain reaction[J]. Journal of Chongqing Medical University, 1999,(1).
Zhang Yuhong, et al. Processed pseudogenes interfere with the reliability of reverse tran-scriptase - polymerase chain reaction[J]. Journal of Chongqing Medical University, 1999,(1).DOI：
To find and overcome the interference of processed pseudogene in reverse transcriptase-polymerase chainreaction(RT- PCR). the author aniplified thesequence of p - acting ene in several tissues. with RT - PCR metheod.The infiuence of p-actin processed pseudogene could be found by performing certain controls. Genomic DNAcontaniinatioiis as low as
0.1%
in given RNA samples could givefalse-positive signal. The false-positiveresults generated by processed pseudogene in manimal genome is universal for RT-PCR- By digesting theextracted RNA samples with DNase before cDNA synthesis
the problem could be effectively solved. This is ofgreat importance for promoting the reliaility of RT - PCR.
京公网安备11010802024621