By using the culture of hematopoietic progenitor cell and hematopoietic growth factor(HGF) assay

the effectof total saponins of Panax notoginseng(TSPN) on the proliferation of CFU - GM and is mechanism were studied.The results indicated that TSPN auginentet the proliferation of CFU- GM in vitro or in vivo

that the spleen cellconditioned mediafCSM)

L-cell conditioned media(LcCM) and skeletal muscle conditioned media(SMCM)

prepared by treating the cells and tissue with TSPN

had relatively strong stimulation on the proliferation ofCFU-GM. Our research suggests that TSPN may enhance granulopoiesis and monocytopoiesis by actvatingdirectly and/or indirectly on fibroblast and lymphocyte in hematopoietic inductive microenvironment and othertissue to produce some hematopoietic growth factors microenvironment and other tissue to produce somehematopoietic growth factors (such as

GM- CSF). This may be one of the mehanism for the antianemic effect ofTSPN.