Objective: To study the ability of Cisplatin

Mitoxantrone and Cytosine Arabinoside on inhibiting the proliferation of rabbit lens epithelial cells(RLECs). Methods: Lens epithelium was isolated and cultured

and 2~3 passage RLECs were placed in 96-well flat bottomed tissue culture plates and incubated for 24 hours

then exposed to different concentrations of Cisplatin

Mitoxantrone and Cytosine Arabinoside for 24 and 72 hours and the effect of RLECs were measured with methyl thiazolyl tetrazolium (MTT) assay. The apoptosis of cells were observed with transmission electron microscope (TEM) .The changes of cell period were observed with flow cytometer (FCM). Results: The IC50 of cisplatin and mitoxantrone exposed to RLECs for 24 hours were 38.92μg/ml 、8.92μg/ml and those of 72 hour were 6.71μg/ml、2.04μg/ml respectively. Conclusion: Cisplatin and Mitoxantrone were dose- dependent and time-dependent inhibitors. Both of them inhibited the proliferation of RLECs effectively

Cytosine Arabinoside

however

had no obvious effect on RLECs in this experimental condition.