Objective: To immunize rabbit with the eukaryotic expression plasmid pcDNA3.1-hALR and obtain antiserum against hALR. Methods: The hALR gene was obtained from recombinant vector PET11a-hALR by PCR technique with the primers designed. The hALR gene was subcloned into the eukaryotic expression αplasmid pcDNA3.1. The recombinant plasmid pcDNA3.1-hALR was obtained and transformed to E.Coli DH5. The resistant clone was selected by ampicillin. The integrity of reading frame of hALR gene in pcDNA3.1-hALR was confirmed through the restriction endonuclease digestion and DNA sequence analysis.Recombinant plasmid pcDNA3.1-hALR and control plasmid pcDNA3.1 were prepared with Midi QIAGEN-Tip-100. DNA contents were measured by ultraviolet quantity at 260nm

respectively. After the injection of these expression plasmids into tibialis anterior of rabbit(per 1.08mg )

the antiserum was obtained. The antiserum was assayed by Western blot. Results: It was constructed successfully for the eukaryotic expression plasmid pcDNA3.1-hALR.After the recombinant plasmid pcDNA3.1-hALR and control plasmid pcDNA3.1 were injected into rabbits respectively

it was confirmed by Western blot that the expression of plasmid pcDNA3.1-hALR induced the production of antibody against hALR. Conclusion: These results suggested that recombinant vector pcDNA3.1-hALR can express the hALR in vivo and stimulate the anti-hALR antibody production. This provides a means for us to study further its biological activity and action mechanism.