恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究

金先庆; 张喜平; 王珊; 许嘉陵

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恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究

更新时间：2024-01-17

- 恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究
- Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer
- 重庆医科大学学报 2002年第4期页码：376-378
- 作者机构：
  
  1. 重庆医科大学儿科学院外科
  2. 重庆医科大学儿科学院外科,重庆,400014
- 作者简介：
- 基金信息：
  
  国家自然科学基金（ 30070781）
- DOI：
  中图分类号： R730.5
- 纸质出版日期：2002，
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[1]金先庆,张喜平,王珊,许嘉陵.恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究[J].重庆医科大学学报,2002(04):376-378+382.

JIN Xianqing, et al. Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer[J]. Journal of Chongqing Medical University, 2002,(4):376-378.
[1]金先庆,张喜平,王珊,许嘉陵.恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究[J].重庆医科大学学报,2002(04):376-378+382. DOI：

JIN Xianqing, et al. Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer[J]. Journal of Chongqing Medical University, 2002,(4):376-378. DOI：

摘要

目的：本研究通过外周血单个核细胞（PBMC）、CD4+、CD8+细胞P-糖蛋白的表达与肿瘤组织P-糖蛋白表达的相关性研究以及PBMC、CD4+、CD8+细胞P-糖蛋白在化疗过程中的动态变化的观察，寻找动态诊断MDR的新途径。方法：本组共51例肿瘤病人，23例采用免疫组化检测PBMC及肿瘤组织的P-糖蛋白，28例用流式细胞术测定PBMC、CD4+、CD8+ 细胞P-糖蛋白，用免疫组化测定相应的肿瘤组织的P-糖蛋白。结果：①肿瘤患者PBMC、CD4+、CD8+细胞P-糖蛋白表达较正常人高（P＜ 0.05）。②PBMC、CD4+、CD8+细胞P-糖蛋白表达水平与肿瘤组织P-糖蛋白呈显著的相关性（r =0.419

P<0.05）；③PBMC、CD4+P-糖蛋白化疗后较化疗前显著增加，可通过PBMC、CD4+P-糖蛋白的变化动态反映肿瘤细胞的变化。④用FCM检测PBMC P-糖蛋白与IC法比较，FCM方法简单，精确度高，更适合血细胞的检测。结论：PBMC、CD4+、CD8+P-糖蛋白含量与肿瘤P-糖蛋白表达有明显相关性。通过PBMC、CD4+、CD8+P-糖蛋白的检测，为临床判断肿瘤耐药提供了一条简便的途径。

Abstract

Objective: At present

the detection of multidrug resistance depends on resected specimen but it can not be investigated in patients who were not operated on or got a long term chemotherapeutic treatment after operation. In this study

the relationship between p-glycoprotein expression in PBMC，CD 4+，CD8+ and tumor cells has been investigated. In order to obtain a new determination method for multidrug resistance

the changes of p-glycoprotein expression in PBMC，CD 4+，CD 8+ during chemotherapeutic stage has been analysed. Methods: The expression of p-glycoprotein in PBMC，CD 4+，CD8+ and tumor cells has been detected by means of immunohistochemestry assay in 23 cases

flow cytometry and immunohistochemestry assay in 28 cases to identify the relationship of p-glycoprotein between PBMC，CD 4+，CD8+ and tumor cells. P-glycoprotein in PBMC，CD 4+ and CD8+ was measured in 17 of 28 patients with chemotherapeutic treatment for six months. Results: ①The p-glycoprotein expression in PBMC，CD4+，CD8+ of patient with malignant tumor was higher than the control. ②A significance relationship of p-glycoprotein had been found between PBMC，CD 4+，CD8+ and tumor cells. ③ The p-glycoprotein expression in PBMC，CD 4+，CD 8+ increased significantly in chemotherapeutic group as compared with non-chemotherapeutic group. ④Flow cytometry assay was more sensitive than immunohistochemestry in detecting p-glycoprotein expression in PBMC，CD 4+，CD8+ . Conclusion:The detection of p-glycoprotein in PBMC，CD 4+，CD8+ provides a new method in the MDR determination. A relationship of p-glycoprotein has been found between PBMC，CD 4+，CD8+ and tumor cells.

关键词

多药耐药外周血单个核细胞肿瘤免疫组化流式细胞术

Keywords

Peripheral blood mononuclear cellMultidrug resistancetumorFlow cytometryImmunohisto-chemistry

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