YU Lurong, et al. Determination of triptolide in the sustained-release capsule ofTripterygium Wilfordii Hook.f.with HPLC[J]. Journal of Chongqing Medical University, 2006,(1):117-118.
YU Lurong, et al. Determination of triptolide in the sustained-release capsule ofTripterygium Wilfordii Hook.f.with HPLC[J]. Journal of Chongqing Medical University, 2006,(1):117-118.DOI:
Objective: To establish a method for content determination of triptolide in the sustained-release capsule of Tripterygium Wilfordii by high performance liquid chromatography.Methods: High performance liquid chromatography was performed on C18 column.The chromatographic conditions were as follows: Acetonitrile-water(40:60) as mobile phase;flow rate being 1.0 ml/min and the detecting wavelength at 220nm.Results:Triptolide had good linear relation within the range from 0.047 to 0.350μg/ml(r=0.9995).The average recovery was 99.88%(RSD=0.58%
n=5).Conclusion: This method is convenient
accurate
reproducible
which can be used for content determinaton of triptolide.