Objective: By hybrid technology

to study the biological characteristics and antitumor function of gastric cancer-dendritic fusion cells through the fusion of dendritic cells from cord blood with gastric cancer cells SGC7901

Method: DCs from cord blood were fused with SGC7901 in the presence of 50% polyethylene glycol.The morphology and phenotype of DCs

SGC7901 and DCs/SGC7901 were respectively observed by SP immunocytechemical staining.The ability of DCs/SGC7901 which stimulated proliferation of autologous T lymphocytes was assessed by MTT

and the kill rate of cytotoxic T lymphocytes(CTL) to SGC7901 induced by FCs(fusion cells) was evaluated through cytotoxic test.Result: DCs were successfully fused with SGC7901 in the presence of PEG

FCs expressed CEA and HLA-DR two phenotypes.Moreover

fusion cells had greater ability to stimulate proliferation of T lymphocytes than DCs?DCs+SGC7901

SGC7901

and the kill rate of FCs(21.4%±0.0165%) was greatty high than that of DCs(16.3%±0.0015%)

DCs+SGC7901(15.3%±0.0044%)

SGC7901(11.2%±0.0156%).Conclusion: DCs from cord blood can be successfully fused with SGC7901 in the presence of PEG

and FCs can effectively stimulate T lymphocytes to elicit antitumor immunity effect.