Objective: To investigate the influence of ischemic and pinacidil preconditioning on the activity of NF-κB and its myocardial protective effects.Methods: Eighty isolated rabbit hearts were randomized into five groups(Group C?K?I?P?G).Hearts were removed and mounted in a Langendorff apparatus perfused with 37℃ oxygenated Krebs-Henselet buffer.All groups were subjected to gobal myocardial ischemia for 40 min and reperfusion for 60 min or 120 min.Cardiac functions were monitored at 10 min equilibration ?15 min?30 min?60 min and 120 min reperfusion;Coronary flow(CF) was measured at 10 min equilibration and 30 min reperfusion;Expression of NF-κB P65 was determined at 10 min equilibration?60 min and 120min reperfusion.Results:(1)After ischemic reperfusion

the recovery of cardiac function and CF in Group I and Group P was significantly superior to that in Group C and Group K(P<0.01 or P<0.05);(2) The expression of NF-κB P65 in Group I and Group P was greater than that in Group C and Group P;The expression of NF-κB P65 in Group G were higher than that in Group P(P<0.05).Conclusion: Both ischemic and pinacidil preconditioning can protect myocardium from myocardial inflammation during ischemic reperfusion

the mechanisms of which may be that the opening sensitive potassium channel might have inhibited the activation of NF-κB directly or indirectly.