Objective:To investigate the expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro and the mechanism of anti-invasion.Methods:The protein expression level of HSP90 in ZR-75-30 before and after treatment with HSP90 inhibitor Geldanamycin was detected by Western-blotting;The effects of GA on the proliferation

invasion and motility of human breast cancer cell line ZR-75-30 in vitro were explored respectively by MTT colorimetric assay and transwell chamber.Results:After treatment with GA the protein expression level of HSP90 in ZR-75-30 was decreased

and the proliferation of ZR-75-30 cells was obviously inhibited in a dose and time-dependent manner.Compared with the control guoup

the adhesion

invasion and motility abilities of the ZR-75-30 cells were decreased significantly(P<0.01).Conclusion:The results showed that The proliferation and invasive ability of the human breast cancer cell line ZR-75-30 can be suppressed by Geldanamycin and heat shock protein(HSP)90 is associated with the invasive and metastatic abilities of breast cancer cell.