Objective:To establish and assess a laparoscopic pneumoperitoneum model in vitro.Methods:The model was established by a special airtight container made of stainless steel and an automatic pneumoperitoneum meter.After the cervical carcinoma cell line(CaSki) was exposed to 100% carbon dioxide in this model

the effect of carbon dioxide pneumoperitoneum on the cell growth was determined with MTT assay.And the experiment was repeated for three times.Results:There was no significant difference of the absorbance values at 24h?48h after exposure between the two groups(P>0.05)

but at 72h

96h

120h after exposure

the absorbance values in carbon dioxide pneumoperitoneum group were always significantly higher than those in control group at the same time(P<0.05).There was no significant difference of the change of the absorbance values either in the carbon dioxide pneumoperitoneum group or in the control group among three times(P>0.05).Conclusions:The model established with a special airtight container and an automatic pneumoperitoneum meter is an ideal one for research about laparoscopy in vitro

and the carbon dioxide pneumoperitoneum in this model increases the in vitro growth of human cervical carcinoma cells.