Objective:To explore the feasibility and stability of using oleic acid to establish acute respiratory distress syndrome(ARDS) model in Beagle dogs.Methods:A total of 25 Beagle dogs were injected oleic acid with a speed of 0.25±0.03ml/Kg through jugular vein after being anesthetized.The gas ventilation index

oxygen metabolism

histopathological changes

chest-ray scan and stability of each index after establishing the ARDS situation were continuously monitored and evaluated.Results:All 25 dogs were reached the diagnosis criteria of ARDS in 4.16±0.92 hours after injection of oleic acid

The arterial PaCO2 of the animals was 27.98±8.25mmHg

PaO2 was 65.40±11.48mmHg

and PaO2/FiO2 was 182.3±29.6.After mechanical ventilation treatment

PaO2/FiO2≤200.Pulmonary mesenchyme

and alveolus edema

bleeding

shrinkage and transparent formation were seen under microscope.There was darkness or coarse shadow in the lung by the X-ray scan.Conclusions:Through injection of oleic acid via jugular vein

a Beagle dog ARDS model may be quickly established with stable changes of hemodynamics

pulmonary mechanics and histology.