Objectve: To evaluate the effects of pigment epithelium derived factor(PEDF) eyedrops in inhibiting corneal neovascularization(CNV) by topical installition on the rat model undergoing penetrating keratoplasty and to investigate its possible mechanism and its clinical significance.Methods: Penetrating karatoplasty were proformed orthotopically from Wistar rats to SD rat’ recipients.72 SD rats were randomly assigned to two groups-experimental group and control group

after transplantation.Experimental group was treated with PEDF eyedrops and control group was treated with escipient eyedrops.CNV was observed by the slitlamp biomicroscope examination.The area of CNV was caculated quantitatively.6 animals in both groups were killed respectively at the 1st week

2nd week

3rd week

1st month

2nd month and 3rd month postoperatively.PEDF expression was examined by immunohistochemistry and corneal untrastructural morphology was observed by TEM.Results: The area of CNV was decreased at 1 week

2 weeks

3 weeks

1 month and 2 months postaperatively(P<0.01 or P<0.05).Significantly increased PEDF expression was also observed in the experimental group by immunohistochemistry staining

as compared with the control group.Conclusion: Topical application of PEDF eye drop can prevent vascular growth of corneal allografts.